GATA-4 gene organization and analysis of its promoter
GATA-4 gene organization and analysis of its promoter
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DOI：
10.1248/bpb.29.410
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发表时间：
2006-03-01
影响因子：
2
通讯作者：
Maeda, M
中科院分区：
文献类型：
--
作者：
Ohara, Y;Atarashi, T;Maeda, M
小鼠GATA-4基因由6个内含子隔开，这种基因组织在啮齿动物和人类中都是保守的。GATA-4基因在大鼠心脏、胃和睾丸以及表达GATA-4的培养细胞如TM3、TM4、1-10和P19.CL6细胞中的转录起点基本相同。GATA-4基因的5‘上游在啮齿动物和人类中也是保守的。我们利用睾丸来源的TM3和TM4细胞，通过荧光素酶报告基因检测其启动子活性。位于GATA-4基因转录起始点上游几十个碱基对的GC-box和E-box对GATA-4基因的启动子活性起关键作用，这与非TATA启动子的转录特征一致。在DMSO的诱导下，CL6细胞分化为搏动的心肌细胞，并伴随着包括GATA-4在内的心脏特异基因转录的刺激。有趣的是，它们在DMSO诱导下表现出更高的荧光素酶报告基因活性。在P19.CL6细胞中，近端串联GC盒和E盒也参与了报告基因的活性。
The mouse GATA-4 gene is separated by six introns, and this gene organization is conserved in rodents and man. The transcriptional start site of the GATA-4 gene is essentially the same in rat heart, stomach and testis, and in cultured cells expressing GATA-4 such as TM3, TM4, 1-10 and P19.CL6 cells. The 5'-upstream of the GATA-4 gene is also conserved in rodents and man. We examined its promoter activity by means of luciferase reporter gene assay using testis-derived TM3 and TM4 cells. The GC-boxes and E-box located in the several tens of base pairs upstream of the transcriptional start sites of the GATA-4 gene were found to be critical for its promoter activity in these cells, consistent with the mode of transcription characteristics of the TATA-less promoter. P19.CL6 cells differentiate into beating cardiomyocytes upon induction by DMSO, accompanied by stimulation of the transcription of heart-specific genes including GATA-4. Interestingly, they exhibit increased luciferase reporter gene activity upon induction by DMSO. Both proximal tandem GC-boxes and the E-box are also contributed to the reporter gene activity in P19.CL6 cells.