STOP-FLOW - NEW TECHNIQUE FOR MEASURING AXONAL-TRANSPORT, AND ITS APPLICATION TO TRANSPORT OF DOPAMINE-BETA-HYDROXYLASE
STOP-FLOW - NEW TECHNIQUE FOR MEASURING AXONAL-TRANSPORT, AND ITS APPLICATION TO TRANSPORT OF DOPAMINE-BETA-HYDROXYLASE
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DOI：
10.1002/neu.480060404
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发表时间：
1975-01-01
期刊：
影响因子：
--
通讯作者：
BRIMIJOIN, S
中科院分区：
文献类型：
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作者：
BRIMIJOIN, S
设计了一种利用局部冷却可逆地阻断离体兔坐骨神经多巴胺-β-羟化酶（DBH）轴突运输的装置，将神经短区域的温度降低到1 ~ 3°C，而将其余区域保持在37°C，引起DBH活性在冷却区域及其近端积聚。这种蓄积在冷却0.5小时后明显，并在约3小时内随时间以几乎线性的方式增加。当神经复温至37°C时，冷却诱导的运输中断迅速逆转。局部冷却1.5小时后复温，DBH活性峰值以300 ± 17 mm/天的速度向神经远端迁移。这个速度被保持，只要可以遵循的峰值，是四倍以上的平均速度估计的DBH活动的积累率以上的结扎在这些相同的神经的远端。它的结论是，结扎实验严重低估了DBH的轴突运输的真实速度，本技术提供了很大的优势，允许直接研究运输材料的单独轴突隔室的迁移。
An apparatus was devised which utilizes local cooling to reversibly interrupt the axonal transport of dopamine‐β‐hydroxylase (DBH) in rabbit sciatic nervesin vitro.Lowering the temperature of a short region of nerve to between 1 and 3°C, while keeping the remainder at 37°C, caused DBH activity to accumulate in and proximal to the cooled region. This accumulation was evident after 0.5 hr of cooling and increased in a nearly linear fashion with time for about 3 hr. The cooling‐induced interruption in transport was rapidly reversed when nerves were rewarmed to 37°C. Upon rewarming after local cooling for 1.5 hr, a peak of accumulated DBH activity migrated toward the distal end of the nerve at a velocity of 300 ± 17 mm/day. This velocity was maintained for as long as the peak could be followed and was four times greater than the average velocity estimated from the rate of accumulation of DBH activity above a ligature at the distal end of these same nerves. It is concluded that ligation experiments grossly underestimate the true velocity of axonal transport of DBH and that the present technique offers great advantages in permitting direct study of the migration of separate axonal compartments of transported materials.