Gender differences of oligomers and total adiponectin during puberty: A cross-sectional study of 859 Danish school children
Gender differences of oligomers and total adiponectin during puberty: A cross-sectional study of 859 Danish school children
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DOI：
10.1210/jc.2006-2310
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发表时间：
2007-05-01
影响因子：
5.8
通讯作者：
Flyvbjerg, Allan
中科院分区：
文献类型：
--
作者：
Andersen, Kristian Kjaer;Frystyk, Jan;Flyvbjerg, Allan
背景：青春期阶段已被证明影响总脂联素（ADPN）水平。目的：本研究的目的是探讨青春期阶段和睾酮水平是否影响血清总ADPN水平和ADPN异构体的分布。设计：这是一项横断面研究。患者：本研究包括859名儿童和青少年（396名男性），年龄6-20岁。主要结果测量：总ADPN和ADPN异构体的测量使用一个有效的内部免疫荧光测定。在青春期前和青春期后男性和女性的代表性亚组中，将ADPN分为三个主要分子组分（n = 40，每组10个）使用经验证的快速蛋白质液相色谱法。青春期前男性和女性的总ADPN水平分别为13.4（11.1-15.9）mg/L（中位数和四分位数间距）和14.7（12.3-18.1）mg/L（P =不显著）。青春期后，男性ADPN水平显著降低，为9.7（8.2-12.0）mg/L，但女性保持不变，为12.1（9.7-15.3）mg/L（P < 0.0001）。同时，当比较青春期前和青春期后男性时，观察到高分子量（HMW）异构体与总ADPN的比值（HMW比值）降低。此外，青春期后男性的HMW比值低于相应的女性（P = 0.038）。血清总ADPN水平与睾酮水平呈负相关（r =-0.430，P = 0.007）。此外，在青春期开始时，在雄性动物中观察到HMW比值降低。我们推测，高分子量ADPN的抑制可能是由睾酮引起的。
Context: Pubertal stages have been shown to influence total adiponectin ( ADPN) levels. Furthermore, testosterone has been shown to alter the isomer distribution of ADPN.Objective: The goal of this study was to investigate whether pubertal stages and testosterone levels influenced total serum ADPN levels and the distribution of ADPN isomers.Design: This is a cross-sectional study.Patients: The study included 859 children and adolescents ( 396 males) aged 6-20 yr.Main Outcome Measures: Total ADPN and ADPN isomers were measured using a validated in-house immunofluorometric assay. Fractioning of the ADPN into the three major molecular fractions was performed in representative subgroups of pre- and postpubertal males and females ( n = 40, 10 in each group) using a validated fast protein liquid chromatography method.Results: Total ADPN levels before puberty were 13.4 ( 11.1-15.9) mg/liter ( median and interquartile range) and 14.7 ( 12.3-18.1) mg/liter ( P = not significant), in males and females, respectively. After puberty, ADPN levels were significantly reduced in males, 9.7 ( 8.2-12.0) mg/liter but remained unchanged in females, 12.1 ( 9.7-15.3) mg/liter ( P < 0.0001). Concomitantly, a reduction was seen in the ratio of high-molecular-weight ( HMW) isomers to total ADPN ( HMW ratio) when comparing prepubertal and postpubertal males. Also, postpubertal males had lower HMW ratios than corresponding females ( P = 0.038). Finally, a negative correlation was seen between HMW ratio and testosterone ( r = -0.430, P = 0.007).Conclusion: Serum total ADPN levels decrease through puberty in males. Also, a reduced HMW ratio is seen in males at the onset of puberty. We speculate that the suppression of HMW ADPN may be caused by testosterone.