Suppression of Cell-cycle Progression during the S Phase of Rat Fibroblasts by 3-Deoxyglucosone, a Maillard Reaction Intermediate
Suppression of Cell-cycle Progression during the S Phase of Rat Fibroblasts by 3-Deoxyglucosone, a Maillard Reaction Intermediate
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美拉德反应中间体 3-脱氧葡萄糖酮对大鼠成纤维细胞 S 期细胞周期进程的抑制
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发表时间：
1994
期刊：
影响因子：
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通讯作者：
H. Kato
中科院分区：
文献类型：
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作者：
T. Shinoda;F. Hayase;H. Kato
3-脱氧葡萄糖苷(3DG)是蛋白质与葡萄糖美拉德反应的主要中间产物，通过抑制DNA合成来抑制多种细胞系的增殖。我们从细胞周期进程的角度探讨了抑制细胞增殖的机制。3Y1细胞培养中加入3DG后，大鼠成纤维细胞呈指数生长期，加入300或600μg/ml3DG后，细胞明显停滞于G1期和G2/M期。我们观察到，在3Y1细胞中，3DG特异性地抑制了从早期S期释放出来的同步培养的S期的时间依赖性进展。3DG对从GO期释放的细胞有影响，但对GO期滞留细胞无影响。用还原型谷胱甘肽(GSH)合成酶抑制剂降低3Y1细胞内还原型谷胱甘肽(GSH)浓度时，3DG对[~3H]TdR掺入的抑制作用增强。因此，我们假设那个细胞...
3-Deoxyglucosone (3DG), the main intermediate compound in the Maillard reaction of proteins with glucose, suppressed the proliferation of various cell lines by inhibition of DNA synthesis. We investigated the mechanism of the suppression of cell proliferation from the standpoint of the progression of cell cycle. When 3DG was added to the culture of 3Y1 cells, rat fibroblasts, growing in exponential phase, the addition of 300 or 600μg/ml of 3DG increased the numbers of the cells apparently arrested at the G1 or G2/M phase, respectively. We observed that 3DG specifically inhibited the time-dependent progression during the S phase of a synchronous culture released from the early S phase in 3Y1 cells. 3DG influenced the cells released from the GO phase but not the GO-arrested cells. When an intracellular concentration of reduced glutathione (GSH) in 3Y1 cells was decreased by using a GSH synthetase inhibitor, the inhibition of [3H]thymidine incorporation by 3DG was enhanced. Therefore, we assumed that the cel...