Probiotic treatment of rat pups normalises corticosterone release and ameliorates colonic dysfunction induced by maternal separation
Probiotic treatment of rat pups normalises corticosterone release and ameliorates colonic dysfunction induced by maternal separation
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DOI：
10.1136/gut.2006.117176
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发表时间：
2007-11-01
期刊：
影响因子：
24.5
通讯作者：
Perdue, Mary H.
中科院分区：
文献类型：
--
作者：
Gareau, Melanie G.;Jury, Jennifer;Perdue, Mary H.
背景：我们之前的研究表明，大鼠幼崽的新生儿母分离（MS）会引起肠道生理的即时和长期变化。目的：探讨益生菌是否影响ms诱导的肠道功能障碍。方法：第4 ~ 19天，MS幼崽与坝体分离3 h/d；未分离（NS）幼崽作为对照组。分离期间，分别给MS和NS幼崽108种益生菌（2株乳酸菌），每天2次；给药的幼崽注射生理盐水。在第20天进行研究，收集血液进行皮质酮测量，作为下丘脑-垂体-肾上腺（HPA）轴活性的指示，并在Ussing腔中研究组织的结肠功能。离子输运用基线和受激短路电流（Isc）表示；用辣根过氧化物酶（HRP）在结肠组织中的通量测定大分子通透性；通过选择培养基培养组织，定量细菌对粘膜的粘附/渗透。在第60天对结肠功能和宿主防御进行评估。结果：MS犬结肠Isc和HRP通量明显高于NS犬。MS幼崽中总细菌的粘附/渗透增加，但乳酸杆菌种类显著减少。在第20天和第60天，益生菌给药改善了ms诱导的肠道功能异常和细菌粘附/渗透，并降低了第20天升高的皮质酮水平。结论：肠道菌群的改变与结肠病理生理有关。益生菌改善多发性硬化症引起的肠道功能障碍，至少部分是通过HPA轴活性的正常化。
Background: We previously showed that neonatal maternal separation (MS) of rat pups causes immediate and long-term changes in intestinal physiology.Aim: To examine if administration of probiotics affects MS-induced gut dysfunction.Methods: MS pups were separated from the dam for 3 h/day from days 4 to 19; non-separated (NS) pups served as controls. Twice per day during the separation period, 108 probiotic organisms (two strains of Lactobacillus species) were administered to MS and NS pups; vehicle-treated pups received saline. Studies were conducted on day 20, when blood was collected for corticosterone measurement as an indication of hypothalamus-pituitary-adrenal (HPA) axis activity, and colonic function was studied in tissues mounted in Ussing chambers. Ion transport was indicated by baseline and stimulated short-circuit current (Isc); macromolecular permeability was measured by flux of horseradish peroxidase (HRP) across colonic tissues; and bacterial adherence/penetration into the mucosa was quantified by culturing tissues in selective media. Colonic function and host defence were also evaluated at day 60.Results: Isc and HRP flux were significantly higher in the colon of MS versus NS pups. There was increased adhesion/penetration of total bacteria in MS pups, but a significant reduction in Lactobacillus species. Probiotic administration ameliorated the MS-induced gut functional abnormalities and bacterial adhesion/penetration at both day 20 and 60, and reduced the elevated corticosterone levels at day 20.Conclusions: The results indicate that altered enteric flora are responsible for colonic pathophysiology. Probiotics improve gut dysfunction induced by MS, at least in part by normalisation of HPA axis activity.