Restricted expression and retinoic acid-induced downregulation of the retinaldehyde dehydrogenase type 2 (RALDH-2) gene during mouse development
Restricted expression and retinoic acid-induced downregulation of the retinaldehyde dehydrogenase type 2 (RALDH-2) gene during mouse development
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DOI：
10.1016/s0925-4773(96)00653-3
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发表时间：
1997-02-01
影响因子：
2.6
通讯作者：
Dolle, P
中科院分区：
文献类型：
--
作者：
Niederreither, K;McCaffery, P;Dolle, P
2 型视黄醛脱氢酶 (RALDH-2) 被确定为早期胚胎中主要的视黄酸生成酶。在这里，我们报告了小鼠胚胎发生过程中 RALDH-2 基因的表达域，这可能表明内源性视黄酸 (RA) 合成的区域。在早期原肠胚形成过程中，RALDH-2 在邻近结和原条的中胚层中表达。在头折阶段，中胚层表达仅限于后部区域直至头折底部。随后，RALDH-2 在未分化的体节和视泡中短暂表达，并且沿着胚胎内体腔的侧壁和后肠憩室周围更持久地表达。分化肢体中的 RALDH-2 表达域（包括假定的指间区域）与 RA 诱导型 RAR β 基因的表达域一致，但稍先于其。 RALDH-2 基因还在发育中的头部的特定区域表达，包括牙芽、内耳、脑膜和垂体以及多个内脏。在胚胎第 8.5 天施用致畸剂量的 RA 会导致胚胎尾部区域的 RALDH-2 转录物水平下调，并且可能反映了 RA 合成的负反馈调节机制。 (C) 1997 爱思唯尔科学爱尔兰有限公司
Retinaldehyde dehydrogenase type 2 (RALDH-2) was identified as a major retinoic acid generating enzyme in the early embryo. Here we report the expression domains of the RALDH-2 gene during mouse embryogenesis, which are likely to indicate regions of endogenous retinoic acid (RA) synthesis. During early gastrulation, RALDH-2 is expressed in the mesoderm adjacent to the node and primitive streak. At the headfold stage, mesodermal expression is restricted to posterior regions up to the base of the headfolds. Later, RALDH-2 is transiently expressed in the undifferentiated somites and the optic vesicles, and more persistently along the lateral walls of the intraembryonic coelom and around the hindgut diverticulum. The RALDH-2 expression domains in differentiating limbs, which include presumptive interdigital regions, coincide with, but slightly precede, those of the RA-inducible RAR beta gene. The RALDH-2 gene is also expressed in specific regions of the developing head, including the tooth buds, inner ear, meninges and pituitary gland, and in several viscera. Administration of a teratogenic dose of RA at embryonic day 8.5 results in downregulation of RALDH-2 transcript levels in caudal regions of the embryo, and may reflect a mechanism of negative feedback regulation of RA synthesis. (C) 1997 Elsevier Science Ireland Ltd.