Essential Guide to Reading Biomedical Papers - Recognising and Interpreting Best Practice
Essential Guide to Reading Biomedical Papers - Recognising and Interpreting Best Practice
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阅读生物医学论文的基本指南 - 识别和解释最佳实践
DOI：
10.1002/9781118402184.ch13
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Molnár E
中科院分区：
文献类型：
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作者：
Molnár E
在免疫细胞化学和免疫组织化学技术中，抗体用于特异性标记细胞抗原（例如蛋白质）。这些定位方法从根本上依赖于抗体-抗原相互作用的高特异性、亲和力和敏感性。抗体可直接或间接（通常通过二抗）进行可视化，染色剂可在光学或电子显微镜下轻松检测到。理想的染色剂是稳定的，能够准确地揭示抗原的分布，并且适合低分辨率和高分辨率的定位研究。按照惯例，组织中抗原的免疫化学检测称为免疫组织化学，细胞中的检测称为免疫细胞化学。
In immunocytochemistry and immunohistochemistry techniques, an antibody is used to specifically label a cellular antigen (eg a protein). These localization approaches fundamentally rely on the high specificity, affinity and sensitivity of antibody-antigen interactions. Antibodies are visualized either directly or indirectly (usually via a secondary antibody), with a stain that is easily detectable under a light or electron microscope. An ideal stain is stable, will reveal accurately the distribution of the antigen and is suitable for both low-and high-resolution localization studies. By convention, the immunochemical detection of an antigen in tissues is known as immunohistochemistry, and detection in cells is termed immunocytochemistry.