ADHESION MOLECULE EXPRESSION IN HUMAN SYNOVIAL TISSUE
ADHESION MOLECULE EXPRESSION IN HUMAN SYNOVIAL TISSUE
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DOI：
10.1002/art.1780360203
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发表时间：
1993-02-01
影响因子：
--
通讯作者：
KOCH, AE
中科院分区：
文献类型：
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作者：
JOHNSON, BA;HAINES, GK;KOCH, AE
客观的。我们之前已经表明，E-选择素在类风湿性关节炎（RA）滑膜组织的内皮细胞上表达，因此可能对于将白细胞募集到发炎关节中很重要。在本研究中，我们确定了其他细胞粘附分子，包括选择素以及整合素和免疫球蛋白超基因家族的成员，是否在冷冻滑膜中表达。方法。我们采用免疫组织化学染色来确定 CD31 (PECAM)、CD44（透明质酸受体）、CD62（P-选择素）、Leu-8（L-选择素）和整合素亚基 alpha5 (VLA-5)、alpha6 (VLA-6)、beta1 (VLA 1-6) 和 beta3（玻连蛋白受体）的分布， 来自 9 名 RA 和 9 名骨关节炎 (OA) 患者以及 3 名正常 (NL) 受试者的滑膜组织。结果。 P-选择素在所有检查的滑膜组织的血管内皮上表达。 L-选择素和α5-整合素虽然在多种细胞类型上表达，但在RA滑膜组织上没有差异表达。整合素亚基 α6 和 β1 在某些 RA 滑膜组织成分中下调。相反，CD31 在 RA 上的表达程度高于 OA 衬里细胞和巨噬细胞 (P < 0.05)。与 NL 相比，CD44 在 RA 或 OA 巨噬细胞、衬里细胞和成纤维细胞上表达程度更高（P < 0.05）。与NL相比，整合素亚基β3在RA滑膜血管上强烈表达（P < 0.05）。结论。整合素 VLA 1-6 以及选择素 P 和 L 的表达在 RA 滑膜组织中并未上调。 CD31 和 CD44 在 RA 巨噬细胞和衬里细胞上表达上调，CD44 在 RA 成纤维细胞上表达上调，β3-整合素在 RA 血管上表达上调。 RA滑膜组织中CD31、CD44和β3整合素的上调可能有助于平衡粘附相互作用，从而促进白细胞在发炎关节中的通过和保留。
Objective. We have previously shown that E-selectin is expressed on endothelium in rheumatoid arthritis (RA) synovial tissues, and hence may be important in recruitment of leukocytes into the inflamed joint. In the present study, we determined whether other cellular adhesion molecules, including selectins and members of the integrin and immunoglobulin supergene families, are expressed in frozen synovium.Methods. We employed immunohistochemical staining to determine the distribution of CD31 (PECAM), CD44 (hyaluronate receptor), CD62 (P-selectin), Leu-8 (L-selectin), and the integrin subunits alpha5 (VLA-5), alpha6 (VLA-6), beta1 (VLA 1-6), and beta3 (vitronectin receptor), in synovial tissue from 9 RA and 9 osteoarthritis (OA) patients, and from 3 normal (NL) subjects.Results. P-selectin was expressed on vascular endothelium in all synovial tissues examined. L-selectin and alpha5-integrin, while expressed on a variety of cell types, were not differentially expressed on RA synovial tissues. Integrin subunits alpha6 and beta1, were down-regulated on some RA synovial tissue components. In contrast, CD31 was expressed to a greater extent on RA than on OA lining cells and macrophages (P < 0.05). CD44 was expressed to a greater extent on RA or OA macrophages, lining cells, and fibroblasts compared with NL (P < 0.05). Integrin subunit beta3 was strongly expressed on RA synovial blood vessels compared with NL (P < 0.05).Conclusion. The expression of integrins VLA 1-6, and selectins P and L is not up-regulated in RA synovial tissues. CD31 and CD44 are up-regulated on RA macrophages and lining cells, CD44 on RA fibroblasts, and beta3-integrin on RA blood vessels. The up-regulation of CD31, CD44, and beta3-integrin in RA synovial tissues may help tip the balance of adhesive interactions toward passage and retention of leukocytes in the inflamed joint.