In Vitro Bone Cell Behavior on Porous Titanium Samples: Influence of Porosity by Loose Sintering and Space Holder Techniques
In Vitro Bone Cell Behavior on Porous Titanium Samples: Influence of Porosity by Loose Sintering and Space Holder Techniques
复制标题
DOI：
10.3390/met10050696
复制
发表时间：
2020-05-01
期刊：
影响因子：
2.9
通讯作者：
Torres, Yadir
中科院分区：
文献类型：
--
作者：
Civantos, Ana;Giner, Merce;Torres, Yadir
各种各样的粉末冶金技术可以生产具有与宿主骨组织相似的机械性能的仿生多孔钛结构。在这项工作中，比较了松散烧结和空间固定器技术这两种常用的冶金技术，以评估孔隙率（含量、尺寸、形态和壁粗糙度）、机械性能（刚度和屈服强度）和体外细胞反应（成肌细胞和成骨细胞的粘附和增殖）的影响。进行这些比较是为了实现用于皮质骨置换的部分多孔植入物的生物力学和双功能行为之间的最佳平衡。细胞粘附（丝状伪足的存在）和铺展在多孔表面和完全致密的基底（无孔对照表面）上均得到促进。由于粗糙度和支架孔径较高，使用 50 vol.% NaCl 空间固定器技术设计的多孔支架样品比采用松散烧结技术获得的样品具有更好的生物活性响应。然而，大且不均匀的孔的存在会损害植入物的机械可靠性。考虑到这两种情况，使用 40 vol.% NH4HCO3 和孔径范围在 100 至 200 μm 之间获得的基质提供了尺寸和强度的平衡优化，以促进体外骨整合。
A great variety of powder metallurgy techniques can produce biomimetic porous titanium structures with similar mechanical properties to host bone tissue. In this work, loose sintering and space holder techniques, two frequently used metallurgical techniques, are compared to evaluate the influences of porosity (content, size, morphology and wall roughness), mechanical properties (stiffness and yield strength) and in-vitro cellular responses (adhesion and proliferation of myoblasts and osteoblasts). These comparisons are made to achieve the best balance between biomechanical and bifunctional behavior of a partial porous implant for cortical bone replacement. Cell adhesion (filopodia presence) and spreading were promoted on both porous surfaces and fully dense substrates (non-porous control surfaces). Porous scaffold samples designed using 50 vol.% NaCl space holder technique had an improved bioactive response over those obtained with the loose sintering technique due to higher roughness and scaffold pore diameter. However, the presence of large and heterogeneous pores compromises the mechanical reliability of the implant. Considering both scenarios, the substrates obtained with 40 vol.% NH4HCO3 and pore size ranges between 100 and 200 mu m provide a balanced optimization of size and strength to promote in-vitro osseointegration.