Nuclear magnetic resonance spectroscopy of skin: predictive correlates for clinical application.
Nuclear magnetic resonance spectroscopy of skin: predictive correlates for clinical application.
复制标题
皮肤核磁共振波谱：临床应用的预测相关性。
DOI：
10.1097/00006534-198801000-00001
复制
发表时间：
1988
影响因子：
3.6
通讯作者：
Chapo,GA
中科院分区：
文献类型：
--
作者：
Cuono,CB;Armitage,IM;Marquetand,R;Chapo,GA
The first application of phosphorous 3 l (" P) and proton (" H) nuclear magnetic resonance (NMR) spec-troscopy to the analysis of the metabolic profiles of skin flaps in a rat model and of human skin grafts is presented. Resonances of adenosine triphosphate (ATP), phospho-creatine (PCr), and inorganic phosphate (P) were identified in" P nuclear magnetic resonance spectra. Reso-nanccs of phosphocreatine, creatine (Cr), and lactate (Lac) were identified in'H nuclear magnetic resonance spectra. The most significant finding was the substantial presence of phosphocreatine as the major high-energy phosphometabolite in mammalian skin, a finding which heretofore has not been widely recognized. An energy shuttle between phosphocreatine and ATP is operative in skin to buffer the fall in ATP during ischemic (anaerobic) insult. Inability to replenish exhausted phosphocreatine reserves predictively correlates with eventual flap necrosis. We have defined and analyzed temporal fluxes in the phosphocreatine-creatine and phosphocreatine plus creatine-lactate ratios by proton nuclear nag-netic resonance. Both are sensitive, accurate, and unam-biguous early prognostic indices of eventual flap outcome. These findings support the concept that the fate of a flap may be established as early as 3 hours after elevation and have laid the groundwork for development and application of noninvasive in vivo nuclear magnetic resonance spectroscopy to the study of skin flaps in animals and humans.Despite continuous improvement in our understanding of the anatomic patterns of cutaneous circulation, skin-flap failure remains a frequent clinical reality. The lack of a uniformly effective method for the treatment of a compromised flap reflects our limited knowledge of the underlying pathophysiology. While aberrations in flap hemodynamics have been studied most
创伤、败血症和休克：治疗的生理学基础
DOI：
--
发表时间：
1988
期刊：
影响因子：
--
作者：
J. Wulsin
通讯作者：
J. Wulsin
创伤、败血症和休克：治疗的生理学基础
DOI：
--
发表时间：
1988
期刊：
影响因子：
--
作者：
G. H. Clowes
通讯作者：
G. H. Clowes