Mesoscopic and microscopic imaging of sensory responses in the same animal.
Mesoscopic and microscopic imaging of sensory responses in the same animal.
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DOI：
10.1038/s41467-019-09082-4
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发表时间：
2019-03-07
影响因子：
16.6
通讯作者：
Charpak, Serge
中科院分区：
文献类型：
--
作者：
Boido, Davide;Rungta, Ravi L;Charpak, Serge
基于血流动力学的成像被广泛用于研究感觉处理。在这里，我们调查了局部神经元和毛细血管反应（双光子显微镜）与快速超声（fUS）和BOLD-fMRI检测到的介观反应的相关程度。使用一个专门的慢性嗅球准备，我们报告说，顺序成像相同的小鼠允许定量比较的气味反应，在微观和介观尺度成像。在这些条件下，功能性充血发生在神经元激活的阈值和fUS-CBV信号可以检测到在单个体素的水平与激活地图根据血流速度而变化。神经元和血管的反应作为气味浓度的函数非线性地增加，而微观和介观血管反应与局部神经元钙线性相关。这些数据确立了介观成像技术报告神经活动的优势和局限性。
Imaging based on blood flow dynamics is widely used to study sensory processing. Here we investigated the extent to which local neuronal and capillary responses (two-photon microscopy) are correlated to mesoscopic responses detected with fast ultrasound (fUS) and BOLD-fMRI. Using a specialized chronic olfactory bulb preparation, we report that sequential imaging of the same mouse allows quantitative comparison of odour responses, imaged at both microscopic and mesoscopic scales. Under these conditions, functional hyperaemia occurred at the threshold of neuronal activation and fUS-CBV signals could be detected at the level of single voxels with activation maps varying according to blood velocity. Both neuronal and vascular responses increase non-linearly as a function of odour concentration, whereas both microscopic and mesoscopic vascular responses are linearly correlated to local neuronal calcium. These data establish strengths and limits of mesoscopic imaging techniques to report neural activity.