Functional excitatory synapses in HEK293 cells expressing neuroligin and glutamate receptors
Functional excitatory synapses in HEK293 cells expressing neuroligin and glutamate receptors
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DOI：
10.1152/jn.00647.2003
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发表时间：
2003-12-01
影响因子：
2.5
通讯作者：
Vicini, S
中科院分区：
文献类型：
--
作者：
Fu, ZY;Washbourne, P;Vicini, S
神经连接素是参与突触形成的关键蛋白，这一发现为在神经元和异源细胞之间诱导功能性突触提供了前所未有的机会。我们借此机会记录的第一次突触电流在人胚肾293（HEK 293）细胞转染neuroligin和N-甲基-D-天冬氨酸或AMPA受体亚单位在与大鼠小脑颗粒细胞共培养。这些电流类似于神经元中记录的突触电流，其衰减动力学由突触后亚基组合决定。虽然神经连接素的表达足以检测功能性突触，但用突触后密度-95/突触相关蛋白-90（PSD-95）共转染HEK 293细胞显著增加电流频率。我们的研究结果支持中枢神经系统突触的形成中的神经连接素的作用，验证了PSD-95允许突触成熟的建议，并提供了一个独特的实验模型来研究分子成分如何决定兴奋性突触的功能特性。
The discovery that neuroligin is a key protein involved in synapse formation offers the unprecedented opportunity to induce functional synapses between neurons and heterologous cells. We took this opportunity recording for the first-time synaptic currents in human embryonic kidney 293 (HEK293) cells transfected with neuroligin and the N-methyl-D-aspartate or AMPA receptor subunits in a co-culture with rat cerebellar granule cells. These currents were similar to synaptic currents recorded in neurons, and their decay kinetics was determined by the postsynaptic subunit combination. Although neuroligin expression was sufficient to detect functional synapses, cotransfection of HEK293 cells with Postsynaptic density-95/synapse-associated protein-90 (PSD-95) significantly increased current frequency. Our results support the central role of neuroligin in the formation of CNS synapses, validate the proposal that PSD-95 allows synaptic maturation, and provide a unique experimental model to study how molecular components determine functional properties of excitatory synapses.