Nano needle with buffering beam for single cell stiffness measurement by nanorobotic manipulators inside ESEM
Nano needle with buffering beam for single cell stiffness measurement by nanorobotic manipulators inside ESEM
复制标题
DOI：
10.1109/nano.2012.6321995
复制
发表时间：
2012-10
期刊：
影响因子：
--
通讯作者：
Yajing Shen;M. Nakajima;Zhan Yang;M. Homma;T. Fukuda
中科院分区：
文献类型：
--
作者：
Yajing Shen;M. Nakajima;Zhan Yang;M. Homma;T. Fukuda
单细胞的力学特性表征对于理解细胞的状态和活性是重要的。本文提出了一种新型的纳米针，用于环境扫描电子显微镜（ESEM）内的单细胞刚度测量。利用纳米加工技术，在原子力显微镜（AFM）悬臂梁上制备了带有缓冲梁的纳米针。当针尖接触单个细胞时，梁将变形。因此，可以基于梁的挠度和单元表面上的压痕来计算单个单元的刚度。在此，选择酵母细胞作为生物样品，并将其放置在环境扫描电镜室内的基底上。纳米针由纳米机器人操纵器驱动，该操纵器具有三个自由度，即X、Y和Z平移。纳米针和单细胞的形变可以直接从环境扫描电镜图像中得到。结果表明，单酵母细胞的刚度约为8.7 MPa，通过使用这种纳米压痕方法。
Single cell's mechanical property characterization is important for the understanding of the cell's condition and activity. In this paper, a novel nanoneedle was proposed for single cell stiffness measurement inside an environmental scanning electron microscope (ESEM). The nanoneedle with a buffering beam was fabricated from AFM (atomic force microscopy) cantilever by nano fabrication technique. The beam will deform when the needle tip touched the single cell. Therefore, the stiffness of the single cell can be calculated based on the beam deflection and the indentation on the cell surface. Here, yeast cell was chosen as the biology sample and put on the substrate inside ESEM chamber. The nanoneedle was driven by a nanorobotic manipulator, which has three degrees of freedom, i.e. X, Y and Z translation. The deformation of the nanoneedle and single cell can be got from the ESEM imaging directly. The result shows that the stiffness of a single yeast cell is around 8.7 MPa by using this nano indentation method.