FINE STRUCTURE OF UTERINE SMOOTH MUSCLE OF RAT UTERUS AT VARIOUS TIME INTERVALS FOLLOWING A SINGLE INJECTION OF ESTROGEN
FINE STRUCTURE OF UTERINE SMOOTH MUSCLE OF RAT UTERUS AT VARIOUS TIME INTERVALS FOLLOWING A SINGLE INJECTION OF ESTROGEN
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DOI：
10.1002/aja.1001230209
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发表时间：
1968-01-01
影响因子：
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通讯作者：
ROTHROCK, M
中科院分区：
文献类型：
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作者：
BO, WJ;ODOR, DL;ROTHROCK, M
本文观察了大鼠皮下注射二丙酸雌二醇（10 μg）后6、12、24、48、72和96小时子宫平滑肌细胞的超微结构。对照组大鼠切除卵巢，但不接受激素治疗。雌激素刺激后不久，肌细胞的超微结构发生了变化。在6小时时，注意到外核膜上的核糖体数量增加，膜外翻到周围的细胞质中。颗粒内质网（格尔）在6 ~ 96 h逐渐增多，扩张程度逐渐增加。在24和48小时，线粒体的数量似乎增加，在稍后的时间间隔，它们的大小增加。高尔基复合体在72和96小时比在更早的时间间隔更突出。在6和12小时观察到游离核糖体的增加，但在24小时发生显著的积累，并持续到96小时。糖原颗粒在24和48小时非常明显，在72和96小时减少。在对照组以及6和12小时时，存在许多吞饮小泡和致密体。在以后的时间间隔内，两者的数量都有所减少。细胞器在24小时开始积累在核极，并延长到96小时。所作的观察提供了额外的信息，子宫平滑肌细胞的精细结构后，雌激素刺激和支持以前的大鼠子宫糖原浓度和RNA合成的生化研究。
The fine structure of smooth muscle cells of the rat uterus was studied at 6, 12, 24, 48, 72 and 96 hours after a single subcutaneous injection of estradiol dipropionate (10 μg). The control rats were ovariectomized, but did not receive hormone treatment. Changes in the ultrastructure of the muscle cells occurred soon after estrogen stimulation. An increase in the number of ribosomes on the outer nuclear membrane and evagination of the membrane into the surrounding cytoplasm was noted at six hours. The amount and dilatation of the granular endoplasmic reticulum (GER) increased gradually from 6 to 96 hours. At 24 and 48 hours the mitochondria appeared to increase in number and at later time intervals they increased in size. The Golgi complex was more prominent at 72 and 96 hours than at the earlier time intervals. An increase in free ribosomes was observed at 6 and 12 hours, but a marked accumulation of them occurred at 24 hours and continued to 96 hours. The glycogen granules were very pronounced at 24 and 48 hours with a reduction at 72 and 96 hours. Many pinocytotic vesicles and dense bodies were present in the controls and at 6 and 12 hours. A decrease in number occurred in both at the later time intervals. Organelles accumulated at the nuclear poles beginning at 24 hours and extending to 96 hours. The observations made provide additional information on the fine structure of uterine smooth muscle cells following estrogen stimulation and support previous biochemical studies on glycogen concentration and RNA synthesis in the rat uterus.