Identification and characterization of Sox genes in the silkworm, Bombyx mori
Identification and characterization of Sox genes in the silkworm, Bombyx mori
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家蚕 Bombyx mori 中 Sox 基因的鉴定和表征
DOI：
10.1007/s11033-010-0468-5
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发表时间：
2011-06-01
影响因子：
2.8
通讯作者：
Lu, Cheng
中科院分区：
文献类型：
--
作者：
Wei, Ling;Cheng, Daojun;Lu, Cheng
Sox基因编码的转录因子家族在后生动物的发育中起重要作用，包括性别决定、胚胎发生、神经发生和骨骼发生。我们在家蚕基因组中发现了Sox基因，并对其进化和表达模式进行了表征。9个Sox基因被注释，可分为5组，B-F。B组的4个Sox基因串联聚集在一条染色体上，这是其他昆虫同源基因的共同特征。调查昆虫中Sox基因高迁移群（HMG）盒内含子数量多样性较低。基于40个不同家蚕品种的基因组，我们发现在驯化和野生家蚕群体中，每个Sox基因编码序列的单核苷酸多态性（SNPs）数量相似。然而，基于基因的检测表明，SoxB3和SoxD可能是在家蚕驯化过程中的正选择下进化的。系统发育分析表明，SoxC、SoxD和SoxF群起源于昆虫辐射前，B群和E群则是在昆虫辐射后通过基因复制进化而来。此外，BmSox21a、BmSoxB3、BmSoxD和BmSoxE显示了阶段、组织或性别依赖的表达模式。这些结果为进一步研究Sox基因在家蚕发育和驯化过程中的功能奠定了基础。
Sox genes encode a family of transcription factors with important roles in metazoan development, including sex-determination, embryogenesis, neurogenesis, and skeletogenesis. We identified Sox genes in the Bombyx mori genome and characterized their evolution and expression patterns. Nine Sox genes were annotated, and could be classified into five groups, B-F. Four Sox genes in the B group were tandemly clustered on one chromosome, a characteristic common to their orthologs in other insects. The intron number in the high-mobility group (HMG) box of Sox genes exhibited low diversity across surveyed insects. Based on 40 different silkworm variety genomes, we found a similar number of single nucleotide polymorphisms (SNPs) in the coding sequences of each Sox gene, for domesticated and wild groups. However, a gene-based examination showed that SoxB3 and SoxD might be evolving under positive selection during silkworm domestication. Phylogenetic analysis showed that SoxC, SoxD, and SoxF originated before the radiation of insects, and groups B and E evolved through gene duplication after the radiation of insects. Furthermore, BmSox21a, BmSoxB3, BmSoxD, and BmSoxE reveal stage, tissue, or sex-dependent expression patterns. These results provide a foundation for further surveying the functions of Sox genes during silkworm development and domestication.