Oral microbial profile discriminates breast-fed from formula-fed infants.
Oral microbial profile discriminates breast-fed from formula-fed infants.
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DOI：
10.1097/mpg.0b013e31826f2bc6
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发表时间：
2013-02
影响因子：
2.9
通讯作者：
Johansson I
中科院分区：
文献类型：
--
作者：
Holgerson PL;Vestman NR;Claesson R;Ohman C;Domellöf M;Tanner AC;Hernell O;Johansson I
Little is known about the impact of diet on the oral microbiota of infants although diet is known to affect the gut microbiota. The aims of the present study were to compare the oral microbiota in breastfed and formula-fed infants, and investigate growth inhibition of streptococci by infant-isolated lactobacilli. 207 mothers consented to participation of their three-month old infants. 146 (70.5%) infants were exclusively and 38 (18.4%) partially breastfed, and 23 (11.1%) were exclusively formula-fed. Saliva from all infants was cultured for Lactobacillus species, with isolate identifications from 21 infants. Lactobacillus isolates were tested for their ability to supress Streptococcus mutans and Streptococcus sanguinis. Oral swabs from 73 infants were analysed by the Human Oral Microbe Identification Microarray (HOMIM) and by q-PCR for Lactobacilius gasseri. Lactobacilli were cultured from 27.8% of exclusively and partially breastfed infants, but not from formula-fed infants. The prevalence of 14 HOMIM detected taxa, and total salivary lactobacilli counts differed by feeding method. Multivariate modelling of HOMIM detected bacteria and possible confounders clustered samples from breastfed infants separately from formula-fed infants. The microbiota of breastfed infants differed based on vaginal or C-section delivery. Isolates of Lactobacillus plantarum, L. gasseri and Lactobacillus vaginalis inhibited growth of the cariogenic S. mutans and the commensal S. sanguinis: L. plantarum > L. gasseri > L. vaginalis. The microbiota of the mouth differs between breastfed and formula-fed three-month-old infants. Possible mechanisms for microbial differences observed include species suppression by lactobacilli indigenous to breast milk.
