A single-cell view of the transcriptome during lateral root initiation in Arabidopsis thaliana
A single-cell view of the transcriptome during lateral root initiation in Arabidopsis thaliana
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DOI：
10.1093/plcell/koab101
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发表时间：
2021-04-03
期刊：
影响因子：
11.6
通讯作者：
Nemhauser, Jennifer L.
中科院分区：
文献类型：
--
作者：
Gala, Hardik P.;Lanctot, Amy;Nemhauser, Jennifer L.
根构型是植物适应性的主要决定因素，并在响应有利和不利的环境刺激的不断修改。除了对主根的影响外，环境还可以改变次生根或侧根的位置、间距、密度和长度。侧根发育是植物器官发生中研究最多的例子之一，但关于其最早的步骤仍有许多未解之谜。在捕捉这些第一个分子事件所面临的挑战之一是，这个过程发生在少数细胞中，时间不可预测。单细胞测序方法提供了分离经历这种命运转变的细胞中发生的特定转录变化的机会。利用这种方法，我们成功地捕获了拟南芥启动侧根原基的转录组，并发现了许多与此过程相关的上调基因。我们开发了一种方法来选择性地抑制靶基因转录的木质部极周细胞侧根起源，并证明了这些目标的表达是需要正常的根发育。我们还发现了细胞亚群的周皮层和内胚层细胞文件，响应侧根启动，强调协调跨细胞文件所需的这种命运的转变。
Root architecture is a major determinant of plant fitness and is under constant modification in response to favorable and unfavorable environmental stimuli. Beyond impacts on the primary root, the environment can alter the position, spacing, density, and length of secondary or lateral roots. Lateral root development is among the best-studied examples of plant organogenesis, yet there are still many unanswered questions about its earliest steps. Among the challenges faced in capturing these first molecular events is the fact that this process occurs in a small number of cells with unpredictable timing. Single-cell sequencing methods afford the opportunity to isolate the specific transcriptional changes occurring in cells undergoing this fate transition. Using this approach, we successfully captured the transcriptomes of initiating lateral root primordia in Arabidopsis thaliana and discovered many upregulated genes associated with this process. We developed a method to selectively repress target gene transcription in the xylem pole pericycle cells where lateral roots originate and demonstrated that the expression of several of these targets is required for normal root development. We also discovered subpopulations of cells in the pericycle and endodermal cell files that respond to lateral root initiation, highlighting the coordination across cell files required for this fate transition.