Localization of periodate-Schiff reactive glycosaminoglycans in semi-thin sections embedded in GMA-Quetol 523-MMA--application of a method for correlative light and electron microscopy of identical sites.
Localization of periodate-Schiff reactive glycosaminoglycans in semi-thin sections embedded in GMA-Quetol 523-MMA--application of a method for correlative light and electron microscopy of identical sites.
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嵌入 GMA-Quetol 523-MMA 的半薄切片中高碘酸盐-希夫反应性糖胺聚糖的定位——应用相同位点的相关光学和电子显微镜方法。
DOI：
10.1046/j.1523-1755.2002.00159.x
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
H. Kushida
中科院分区：
文献类型：
--
作者：
Y. Nagato;T. Mitsui;T. Kushida;H. Kushida
用高碘酸和希夫试剂（PAS）处理包埋组织的半薄切片，用相关的光镜和电镜方法确定PAS反应物的精确定位。用醛混合物固定小块组织标本。脱水后，将嵌段嵌入甲基丙烯酸乙二醇酯（GMA）、Quetol 523、甲基丙烯酸甲酯（MMA）和QCU-1的改性混合物中。通过PAS反应对0.2-0.3微米厚的半薄切片进行染色，然后必要时用苏木精复染。结果发现，PAS反应产物的糖胺聚糖和糖蛋白的特定位点被视为在光学和电子显微镜。在对照实验中，证实了反应的特异性。杯状细胞颗粒与反应物呈良好的对比染色。上皮细胞的基底膜和微绒毛为染色层。在精母细胞中，反应产物分布于高尔基体、顶体囊泡和头帽。结果表明，当包埋基体具有低的电子散射特性时，PAS沉积物变得电子致密。使用这种制备半薄切片的方法，进行了糖胺聚糖定位的比较研究。
A correlative light and electron microscope method in which semi-thin sections of embedded tissue were treated with periodate acid and Schiff's reagent (PAS) was used to determine the precise localization of PAS reactive substance. Small blocks of tissue specimens were fixed with aldehyde mixtures. After dehydration, the blocks were embedded in a modified mixture of glycol methacrylate (GMA), Quetol 523, methyl methacrylate (MMA) and QCU-1. Semi-thin sections, 0.2-0.3 micron thick, were stained by the PAS reaction, followed by counterstaining with hematoxylin if necessary. It was found that PAS reaction products representing the specific sites for glycosaminoglycans and glycoproteins were seen in both light and electron microscopy. In the control experiments the specificity of the reaction was confirmed. The granules of goblet cells were well stained and contrasted by the reaction materials. The basement membrane and the microvilli of the epithelial cells appeared as the staining layer. In the spermatocytes the reaction products were demonstrated in the Golgi apparatus, acrosomal vesicles and head cap. The results indicated that the PAS deposits became electron dense when the embedding matrix had a low electron scattering property. Using this method of preparing semi-thin sections, a comparative study of the localization of glycosaminoglycans was performed.