Label-free oxygen-metabolic photoacoustic microscopy in vivo
Label-free oxygen-metabolic photoacoustic microscopy in vivo
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DOI：
10.1117/1.3594786
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发表时间：
2011-07-01
影响因子：
3.5
通讯作者：
Wang, Lihong V.
中科院分区：
文献类型：
--
作者：
Yao, Junjie;Maslov, Konstantin I.;Wang, Lihong V.
几乎所有的疾病，特别是癌症和糖尿病，都表现出异常的氧代谢。准确测量氧代谢率（MRO（2））有助于基础病理生理学研究，甚至有助于疾病的早期诊断和治疗。目前的技术要么缺乏高分辨率，要么依赖于外源对比度。在这里，我们提出了无标记代谢光声显微镜（mPAM）与小血管分辨率非侵入性量化MRO（2）在体内的绝对单位。mPAM是一种独特的模式，可同时对此类量化所需的所有五个解剖学、化学和流体动力学参数进行成像：组织体积、血管横截面、血红蛋白浓度、血红蛋白氧饱和度和血流速度。热疗，冷冻疗法，黑色素瘤，胶质母细胞瘤在体内纵向成像。与直觉相反，增加MRO（2）不一定会导致缺氧或增加氧提取。事实上，早期癌症被发现是高氧尽管高代谢。(C)2011年，美国光电仪器工程师学会（SPIE）。[DOI 10.1117/1.3594786]
Almost all diseases, especially cancer and diabetes, manifest abnormal oxygen metabolism. Accurately measuring the metabolic rate of oxygen (MRO(2)) can be helpful for fundamental pathophysiological studies, and even early diagnosis and treatment of disease. Current techniques either lack high resolution or rely on exogenous contrast. Here, we propose label-free metabolic photoacoustic microscopy (mPAM) with small vessel resolution to noninvasively quantify MRO(2) in vivo in absolute units. mPAM is the unique modality for simultaneously imaging all five anatomical, chemical, and fluid-dynamic parameters required for such quantification: tissue volume, vessel cross-section, concentration of hemoglobin, oxygen saturation of hemoglobin, and blood flow speed. Hyperthermia, cryotherapy, melanoma, and glioblastoma were longitudinally imaged in vivo. Counterintuitively, increased MRO(2) does not necessarily cause hypoxia or increase oxygen extraction. In fact, early-stage cancer was found to be hyperoxic despite hypermetabolism. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.3594786]