Therapeutic efficacy of Ac-DMQD-CHO, a caspase 3 inhibitor, for rat spinal cord injury
Therapeutic efficacy of Ac-DMQD-CHO, a caspase 3 inhibitor, for rat spinal cord injury
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DOI：
10.1016/j.jocn.2007.06.014
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发表时间：
2008-06-01
影响因子：
2
通讯作者：
Colak, Ahmet
中科院分区：
文献类型：
--
作者：
Akdemir, Osman;Berksoy, Ismail;Colak, Ahmet
我们研究了Ac-DMQD-CHO（一种caspase-3抑制剂）在大鼠模型中脊髓损伤的治疗效果和功能恢复。将30只大鼠随机分为3组，每组10只。在第2组和第3组中，脊髓损伤发生在胸部区域。第3组大鼠用Ac-DMQD-CHO处理。在损伤后24小时和5天，基于组织病理学和TUNEL染色结果评价治疗反应。在治疗期间和治疗后评估神经功能。伤后24小时，光学显微镜检查显示第2组和第3组大鼠出现弥漫性出血性坏死、水肿、血管血栓和多形核白细胞浸润，但第3组的空洞和脱髓鞘不太明显。在该时间点，用Ac-DMQD-CHO处理大鼠显著减少了凋亡细胞的数量。脊髓的创伤性损伤引起细胞凋亡，并且Ac-DMQD-CHO的施用减少细胞凋亡并改善功能结果。(C)2007爱思唯尔有限公司保留所有权利。
We investigated the therapeutic efficacy of Ac-DMQD-CHO, a caspase-3 inhibitor, and functional recovery in spinal cord injury in a rat model. Thirty rats were randomized into three groups of 10 each. In groups 2 and 3, spinal cord trauma was produced in the thoracic region. Group 3 rats were treated with Ac-DMQD-CHO. Treatment responses were evaluated based on histopathological and TUNEL staining findings at 24 h and 5 days post-injury. Neurologic performance was assessed during and following treatment. Twenty-four hours after injury, light microscopy examination revealed diffuse hemorrhagic necrosis, edema, vascular thrombi, and polymorphonuclear leukocyte infiltration in group 2 and 3 rats, but cavitation and demyelinization were less prominent in group 3. At this time point, treatment of the rats with Ac-DMQD-CHO significantly reduced the number of apoptotic cells. Traumatic injury to the spinal cord causes apoptosis and administration of Ac-DMQD-CHO decreases apoptosis and improves functional outcome. (C) 2007 Elsevier Ltd. All rights reserved.