Elimination of Porcine Epidemic Diarrhea Virus in an Animal Feed Manufacturing Facility.
Elimination of Porcine Epidemic Diarrhea Virus in an Animal Feed Manufacturing Facility.
复制标题
消除动物饲料生产设施中的猪流行性腹泻病毒。
DOI：
10.1371/journal.pone.0169612
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Jones CK
中科院分区：
文献类型：
--
作者：
Huss AR;Schumacher LL;Cochrane RA;Poulsen E;Bai J;Woodworth JC;Dritz SS;Stark CR;Jones CK
猪流行性腹泻病毒(PEDV)是第一种引起广泛关注的病毒，与可能通过牲畜饲料或原料传播有关。正在制定排除病原体、防止交叉污染和积极降低饲料和配料致病负荷的措施。然而，到目前为止，研究的重点是化学品或热处理在减少实际饲料中的RNA方面的作用，而没有解决生产设施中可能导致成品饲料持续污染的潜在残留污染。本实验的目的是评估使用标准化方案对受PEDV污染的动物饲料制造设施进行消毒的情况。在生产接种PEDV的猪日粮过程中，在整个设施内收集了环境拭子。为了监测设施对病毒的污染，在1)接种前的基线，2)接种饲料生产后，3)使用季铵-戊二醛混合清洁剂之后，4)使用次氯酸钠消毒液之后，以及5)设施加热至60°C 48小时后收集拭子。去污步骤、表面、类型、区域及其相互作用均影响可检测到的PEDV RNA的数量(P<；0.05)。正如预期的那样，在生产受污染的饲料后，从设备表面收集的所有样本都含有PEDV RNA。此外，从非直接饲料接触表面收集的大多数样品在生产受污染的饲料后也呈PEDV RNA阳性，这强调了粉尘在整个制造设施的病原体交叉污染中所起的潜在作用。使用清洁剂、消毒剂和加热可以有效地减少PEDV基因组物质(P<；0.05)，但不能完全消除它。
Porcine Epidemic Diarrhea Virus (PEDV) was the first virus of wide scale concern to be linked to possible transmission by livestock feed or ingredients. Measures to exclude pathogens, prevent cross-contamination, and actively reduce the pathogenic load of feed and ingredients are being developed. However, research thus far has focused on the role of chemicals or thermal treatment to reduce the RNA in the actual feedstuffs, and has not addressed potential residual contamination within the manufacturing facility that may lead to continuous contamination of finished feeds. The purpose of this experiment was to evaluate the use of a standardized protocol to sanitize an animal feed manufacturing facility contaminated with PEDV. Environmental swabs were collected throughout the facility during the manufacturing of a swine diet inoculated with PEDV. To monitor facility contamination of the virus, swabs were collected at: 1) baseline prior to inoculation, 2) after production of the inoculated feed, 3) after application of a quaternary ammonium-glutaraldehyde blend cleaner, 4) after application of a sodium hypochlorite sanitizing solution, and 5) after facility heat-up to 60°C for 48 hours. Decontamination step, surface, type, zone and their interactions were all found to impact the quantity of detectable PEDV RNA (P < 0.05). As expected, all samples collected from equipment surfaces contained PEDV RNA after production of the contaminated feed. Additionally, the majority of samples collected from non-direct feed contact surfaces were also positive for PEDV RNA after the production of the contaminated feed, emphasizing the potential role dust plays in cross-contamination of pathogen throughout a manufacturing facility. Application of the cleaner, sanitizer, and heat were effective at reducing PEDV genomic material (P < 0.05), but did not completely eliminate it.