Nuclearia pattersoni sp n. (Filosea), a new species of amphizoic amoeba isolated from gills of roach (Rutilus rutilus), and its rickettsial endosymbiont
Nuclearia pattersoni sp n. (Filosea), a new species of amphizoic amoeba isolated from gills of roach (Rutilus rutilus), and its rickettsial endosymbiont
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DOI：
10.14411/fp.2003.030
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发表时间：
2003-09-01
影响因子：
1.6
通讯作者：
Pecková, H
中科院分区：
文献类型：
--
作者：
Dyková, I;Veverková, M;Pecková, H
本文报道了一种两栖动物变形虫新种，PattersoniNuclearisp. n.，分离自红鲤鳃。本文介绍其特征是滋养体细长扁平，形状不规则。它们的身体的较长尺寸为13.2（11.0-15.7）μ m。丝状伪足主要从两极辐射，比身体长2至2.5倍。较少观察到的球形滋养体的直径为8.2-10.8 μ m;它们的丝状伪足向各个方向辐射。囊状阶段有较短的伪足，只从一个极点出现。琼脂平板培养的机车型表面有一层薄的无定形糖萼，而大多数细胞被两层细胞外基质覆盖。线粒体有扁平的嵴，核周区有网质体。形态相似的N.单纯核周纹状带不存在。光镜和超微结构数据与SSU rRNA基因序列和系统发育分析，包括相关类群的序列完成。在N. pattersoni型菌株RR 2G 2被指定为立克次体属。
A new species of amphizoic amoeba, Nuclearia pattersoni sp. n., isolated from gills of Rutilus rutilus L. is described. It is characterised by elongate flattened trophozoites of irregular shape. The longer dimension of their bodies is 13.2 (11.0-15.7) mum. Filopodia radiating mostly from the poles are 2 to 2.5 times longer than the body. The diameter of less frequently observed spherical trophozoites is 8.2-10.8 mum; their filopodia radiate to all directions. Cyst-like stages have shorter pseudopodia that arise from one pole only. The surface of locomotive forms from agar plate cultures has a thin amorphous glycocalyx, while most cells are covered by two layers of extracellular matrix. Mitochondria have flattened cristac, dictyosomes are located in the perinuclear zone. A conspicuous ultrastructural feature of the morphologically similar N. simplex, perinuclear striated band, is not present. Light microscopic and ultrastructural data are completed with the sequence of SSU rRNA gene and phylogenetic analysis including sequences of related taxa. The bacterial endosymbiont found in N. pattersoni type strain RR2G2 is assigned to the genus Rickettsia.