Label-free biodetection using a smartphone
Label-free biodetection using a smartphone
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DOI：
10.1039/c3lc40991k
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发表时间：
2013-01-01
期刊：
影响因子：
6.1
通讯作者：
Cunningham, Brian T.
中科院分区：
文献类型：
--
作者：
Gallegos, Dustin;Long, Kenneth D.;Cunningham, Brian T.
利用其集成的摄像头作为光谱仪，我们演示了使用智能手机作为无标记光子晶体生物传感器的检测仪器。定制设计的支架将智能手机与光学组件固定对准，允许准确和可重复地测量传感器谐振波长的偏移。外部提供的宽带入射到入射针孔的光随后在穿过生物传感器之前被准直和线偏振，生物传感器只共振地反射一小段波长。衍射光栅将剩余的波长分布在相机的像素上，以显示高分辨率的透射谱。该光子晶体生物传感器被制造在塑料衬底上，并附着在标准的玻璃显微镜载玻片上，该载玻片可以很容易地在光路内移除和更换。开发了一个定制软件APP，将相机图像转换为可见光波长范围内的光子晶体透射谱，包括曲线拟合分析，以0.009 nm的精度计算光子晶体的共振波长。我们通过检测固定的蛋白质单层和选择性地检测与功能化光子晶体结合的浓度依赖的抗体来展示该系统的功能。我们设想了一种廉价的、具有网络连接的手持式生物传感器仪器的能力，以在以前不切实际的环境中实现护理点传感。
Utilizing its integrated camera as a spectrometer, we demonstrate the use of a smartphone as the detection instrument for a label-free photonic crystal biosensor. A custom-designed cradle holds the smartphone in fixed alignment with optical components, allowing for accurate and repeatable measurements of shifts in the resonant wavelength of the sensor. Externally provided broadband light incident upon an entrance pinhole is subsequently collimated and linearly polarized before passing through the biosensor, which resonantly reflects only a narrow band of wavelengths. A diffraction grating spreads the remaining wavelengths over the camera's pixels to display a high resolution transmission spectrum. The photonic crystal biosensor is fabricated on a plastic substrate and attached to a standard glass microscope slide that can easily be removed and replaced within the optical path. A custom software app was developed to convert the camera images into the photonic crystal transmission spectrum in the visible wavelength range, including curve-fitting analysis that computes the photonic crystal resonant wavelength with 0.009 nm accuracy. We demonstrate the functionality of the system through detection of an immobilized protein monolayer, and selective detection of concentration-dependent antibody binding to a functionalized photonic crystal. We envision the capability for an inexpensive, handheld biosensor instrument with web connectivity to enable point-of-care sensing in environments that have not been practical previously.