Production of virus-specific antisera using synthetic peptides corresponding to sequences in the yellow fever E protein: brief report.
Production of virus-specific antisera using synthetic peptides corresponding to sequences in the yellow fever E protein: brief report.
复制标题
使用与黄热病 E 蛋白序列相对应的合成肽生产病毒特异性抗血清：简要报告。
作者：
R. Jackson;R. Phillpotts
合成肽已被广泛接受用于表位作图以及作为单克隆抗体和多克隆血清生产的免疫原。合成了与黄热病毒（YF 17D）包膜蛋白（E）一级序列中的区域同源的推定抗原肽，并评价了它们产生对亲本蛋白特异性的多克隆抗血清的能力以及它们与一组E特异性mAb的反应性。抗肽血清在ELISA、Western印迹、中和、血凝抑制和免疫荧光试验中与天然病毒反应。在大多数情况下，反应性血清对原始肽具有特异性。然而，尽管肽选择过程的多样性，我们无法确定任何抗肽血清与真实的YF E蛋白特异性反应。
Synthetic peptides have gained widespread acceptance for use in epitope mapping and as immunogens for monoclonal antibody and polyclonal serum production. Putative antigenic peptides homologous to regions in the primary sequence of the envelope protein (E) of yellow fever virus (YF17D) were synthesized and evaluated for their ability to produce polyclonal antisera specific for the parent protein and for their reactivity with a panel of E-specific mAb. Antipeptide sera were reacted with native virus in ELISA, Western blot, neutralization, hemagglutination-inhibition, and immunofluorescence tests. Reactive sera were in most cases specific for the original peptide. However, despite the diversity of peptide selection processes, we were unable to identify any antipeptide serum that reacted specifically with authentic YF E protein.
DOI：
10.1073/pnas.85.15.5409
发表时间：
1988-08-01
影响因子：
11.1
作者：
TAM, JP
通讯作者：
TAM, JP