Application of the indirect fluorescent antibody assay in the study of malaria infection in the Yangtze River Three Gorges Reservoir, China
Application of the indirect fluorescent antibody assay in the study of malaria infection in the Yangtze River Three Gorges Reservoir, China
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DOI：
10.1186/1475-2875-8-199
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发表时间：
2009-08-13
期刊：
影响因子：
3
通讯作者：
Yang Man-Ni
中科院分区：
文献类型：
--
作者：
Wang Duo-Quan;Tang Lin-Hua;Yang Man-Ni
背景：中国长江三峡工程是世界上最大的建设工程之一。三峡大坝周边地区有间日疟和间日疟流行的历史，但在工程建成前没有疟疾流行的全面数据。本研究旨在为长江三峡库区疟疾感染提供可靠的基线，为今后研究三峡工程对疟疾流行的影响提供参考数据。方法：于2008年5月和10月进行2次地区疟疾感染调查，每隔6个月进行一次。尽可能从包括学校人群在内的一般人群中收集约3600份用于寄生虫诊断的双标本血膜和用于血清学的滤纸血点（使用免疫荧光抗体试验）。结果：同一人群感染后总体阳性应答率（1.40/0.72）约为感染前的2倍。各地区均有15岁以下阳性者。结论：该地区存在一定程度的疟疾感染。需要进一步的研究来确定疟疾经历的时间长短、化疗干预以及该地区主要传播媒介的分布。
Background: China Yangtze Three Gorges Project (TGP) is one of the biggest construction projects in the world. The areas around the Three Gorge Dam has a history of tertian malaria and subtertian malaria epidemic, but there are no overall data about malaria epidemics before the completion of the project. The objective of this study was to get a reliable baseline on malaria infection in the Yangtze River Three Gorges reservoir area and to provide reference data for future studies about the impact of the project on malaria epidemics.Methods: Two surveys of malaria infection were carried out in area, at six-month intervals in May and October 2008. About 3,600 dual specimens blood film samples for parasite diagnosis and filter paper blood spots for serology (using the immunofluorescence antibody test) were collected from the general population, including school populations, whenever possible.Results: The overall percentage of positive response of the same population during post-transmission periods was about twice (1.40/0.72) of that in pre-transmission. Positive individuals under 15 years of age were detected in all the localities.Conclusion: A certain extent of malaria infection existed in this area. Additional studies are needed to determine the length of malaria experience, and chemotherapeutic intervention as well as the distribution of main vectors for transmission in this area.