Water-Soluble BODIPY-Based fluorescent probe for BSA and HSA detection
Water-Soluble BODIPY-Based fluorescent probe for BSA and HSA detection
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DOI：
10.1016/j.molliq.2021.117031
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发表时间：
2022-01-05
影响因子：
6
通讯作者：
Antina, Elena, V
中科院分区：
文献类型：
--
作者：
Ksenofontov, Alexander A.;Bocharov, Pavel S.;Antina, Elena, V
利用光谱方法和分子对接研究了4,4-二氟-1,3,7,8-四甲基-2,6-二硫-4-硼-3a，4 -二氮-s-茚二烯钠盐（BODIPY, BP1）与牛血清白蛋白（BSA）和人血清白蛋白（HSA）的相互作用。结果表明，BP1与BSA和HSA形成稳定的超分子配合物主要是由于特异性相互作用。超分子络合物的形成伴随着BP1在FRET作用下的强荧光猝灭。该效应可用于模型生理液中HSA的定性和定量检测。BSA和HSA的检出限分别为0.26和0.05 mg.mL（-1）。结果表明，BP1荧光反应对BSA和HSA在溶液中同时存在具有选择性。BP1荧光探针成功地用于人尿液中HSA的定性和定量检测。结果表明，基于bp1的荧光传感器可用于微量白蛋白尿及相关疾病的早期检测。(C) 2021 Elsevier B.V.版权所有
Interaction of 4,4-difluoro-1,3,7,8-tetramethyl-2,6-disulfo-4-bora-3a,4a-diaza-s-indacene sodium salt (BODIPY, BP1) with bovine serum albumin (BSA) and human serum albumin (HSA) was investigated using spectral methods and molecular docking. It was shown that BP1 forms stable supramolecular complexes with BSA and HSA predominantly due to the specific interactions. Supramolecular complex formation was accompanied by strong fluorescence quenching of BP1 as a result of FRET. This effect could be used for the qualitative and quantitative detection of HSA in model physiological fluids. The detection limit of BSA and HSA were 0.26 and 0.05 mg.mL(-1), respectively. The selectivity of BP1 fluorescence response to the co-presence of BSA and HSA in solution was demonstrated. The BP1 fluorescent probe was successfully used for the qualitative and quantitative detection of HSA in human urine samples. The obtained results indicated the capability of using BP1-based fluorescent sensors in clinical medicine for early detection of microalbuminuria and concomitant diseases. (C) 2021 Elsevier B.V. All rights reserved.