Expression Analysis Systematic Explorer (EASE) analysis reveals differential gene expression in permanent and transient focal stroke rat models
Expression Analysis Systematic Explorer (EASE) analysis reveals differential gene expression in permanent and transient focal stroke rat models
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DOI：
10.1016/j.brainres.2005.11.090
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发表时间：
2006-02-03
期刊：
影响因子：
2.9
通讯作者：
Ford, BD
中科院分区：
文献类型：
--
作者：
Ford, G;Xu, ZF;Ford, BD
为了更深入地了解缺血性卒中的分子机制，我们使用表达分析系统探索者（EASE）通路分析软件比较了短暂性（tMCAO）和永久性大脑中动脉闭塞（pMCAO）卒中模型的基因表达谱。在两种中风模型中都诱导了许多转录物，包括与转录途径、细胞死亡、应激反应和代谢相关的基因。然而，EASE分析的调节基因表明，分子功能和生物学过程中独特的每一个模型。与tMCAO相关的途径包括炎症、凋亡和细胞周期，而pMCAO与编码神经递质受体、离子通道、生长因子和信号分子的基因的诱导相关。一个有趣的发现是pMCAO后酪氨酸激酶和磷酸酶的参与。这些结果提供了证据表明，tMCAO和pMCAO后的神经元死亡涉及不同的机制。这些发现可能为中风的分子机制提供新的见解，并可能导致新的神经保护策略。(C)2005 Elsevier B.V.保留所有权利。
To gain greater insight on the molecular mechanisms that underlie ischemic stroke, we compared gene expression profiles in transient (tMCAO) and permanent middle cerebral artery occlusion (pMCAO) stroke models using Expression Analysis Systematic Explorer (EASE) pathway analysis software. many transcripts were induced in both stroke models, including genes associated with transcriptional pathways, cell death, stress responses and metabolism. However, EASE analysis of the regulated genes indicated molecular functions and biological processes unique to each model. Pathways associated with tMCAO included inflammation, apoptosis and cell cycle, while pMCAO was associated with the induction of genes encoding neurotransmitter receptors, ion channels, growth factors and signaling molecules. An intriguing finding was the involvement of tyrosine kinases and phosphatases following pMCAO. These results provide evidence that neuronal death following tMCAO and pMCAO involves distinct mechanisms. These findings may give new insight to the molecular mechanisms involved in stroke and may lead to novel neuroprotective strategies. (C) 2005 Elsevier B.V. All rights reserved.