First complete genome sequence of Asia-4 lineage of canine distemper virus in China
First complete genome sequence of Asia-4 lineage of canine distemper virus in China
复制标题
我国首个犬瘟热病毒Asia-4谱系全基因组序列
DOI：
10.1007/s11262-021-01883-4
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发表时间：
2022-01-25
期刊：
影响因子：
1.6
通讯作者：
Bi, Zhenwei
中科院分区：
文献类型：
--
作者：
Bi, Zhenwei
我们之前基于H基因报道了中国一种新型的犬瘟热病毒（CDV）亚洲 - 4谱系。在本研究中，从中国南京分离出一株亚洲 - 4谱系的中国犬瘟热病毒NJ(11)2毒株，并获得了其全基因组序列。在全基因组系统发育树中，CDV NJ(11)2毒株与亚洲 - 4谱系的泰国毒株聚为一类。基于6个单独基因的系统发育分析也表明，CDV NJ(11)2毒株属于亚洲 - 4谱系。根据单独的N基因和H基因，中国的两株毒株，来自新疆的XJ4和来自成都的C11，与亚洲 - 4谱系聚为一类。这些结果表明，犬瘟热病毒的亚洲 - 4谱系出现在中国的三个地区。有趣的是，中国的BJ16B35毒株被鉴定为一种新型的推定重组病毒，其主要亲本病毒为亚洲 - 1谱系，次要亲本病毒为美洲 - 1谱系。中国犬瘟热病毒亚洲 - 4谱系的首个基因组序列有助于了解犬瘟热病毒感染的进化和分子流行病学。
We previously reported a novel Asia-4 lineage of canine distemper virus (CDV) in China based on the H gene. In the present study, a Chinese CDV NJ(11)2 strain of the Asia-4 lineage from Nanjing, China was isolated and its whole genome sequence was obtained. The CDV NJ(11)2 strain clustered with the Thai strains of the Asia-4 lineage in the phylogenetic tree of the complete genome. Phylogenetic analysis based on six individual genes also revealed that the CDV NJ(11)2 strain belonged to Asia-4 lineage. According to the individual N and H genes, two Chinese strains XJ4 from Xinjiang and C11 from Chengdu were clustered with the Asia-4 lineage. These results suggested that the Asia-4 lineage of CDV appeared in the three regions of China. Interestingly, Chinese BJ16B35 strain was identified as a novel putative recombinant virus from a major parent virus of the Asia-1 lineage and a minor parent virus of the America-1 lineage. The first genome sequence of Chinese Asia-4 lineage of CDV contributes to the knowledge of the evolution and molecular epidemiology of CDV infection.