Syndecan-4, a PRRSV attachment factor, mediates PRRSV entry through its interaction with EGFR.
Syndecan-4, a PRRSV attachment factor, mediates PRRSV entry through its interaction with EGFR.
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DOI：
10.1016/j.bbrc.2016.05.084
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发表时间：
2016-06
影响因子：
3.1
通讯作者：
Rui Wang;Xin Wang;Bo Ni;Chang-chao Huan;Jiaqiang Wu;Li-bin Wen;Ying Liao;G. Tong;C. Ding;H. Fan;Xiang Mao
中科院分区：
文献类型：
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作者：
Rui Wang;Xin Wang;Bo Ni;Chang-chao Huan;Jiaqiang Wu;Li-bin Wen;Ying Liao;G. Tong;C. Ding;H. Fan;Xiang Mao
猪繁殖与呼吸综合征的病原体是PRRS病毒（PRRSV），一种有包膜的单链正义RNA病毒。参与PRRSV进入的宿主因素和机制在很大程度上仍然是未知的。在我们目前的研究中，我们发现syndecan-4，硫酸乙酰肝素蛋白多糖之一，在PRRSV进入，特别是在PRRSV附着中起着关键作用。此外，EGFR与多配体蛋白聚糖-4在MACR-145细胞中相互作用，破坏它们的相互作用会损害PRRSV的进入。此外，EGFR抑制剂AG 1478或syndecan-4衍生肽SSTN 87 -131抑制由PRRSV进入诱导的syndecan-4内吞作用。总之，syndecan-4，一种PRRSV附着因子，通过与EGFR相互作用介导PRRSV进入。
The causative agent of porcine reproductive and respiratory syndrome is the PRRS virus (PRRSV), an enveloped, single-stranded and positive-sense RNA virus. The host factors and mechanisms that are involved in PRRSV entry are still largely unknown. In our present studies, we found that syndecan-4, one of the heparan sulfate proteoglycans, plays a critical role in PRRSV entry, especially in PRRSV attachment. Moreover, EGFR interacts with syndecan-4 in MACR-145 cells and disruption of their interaction impaired PRRSV entry. Furthermore, EGFR inhibitor AG1478 or syndecan-4 derived peptide SSTN87-131 inhibited syndecan-4 endocytosis induced by PRRSV entry. Altogether, syndecan-4, a PRRSV attachment factor, mediated PRRSV entry by interacting with EGFR.