Synthesis of fluorescently labeled UDP-GlcNAc analogues and their evaluation as chitin synthase substrates.
Synthesis of fluorescently labeled UDP-GlcNAc analogues and their evaluation as chitin synthase substrates.
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荧光标记的 UDP-GlcNAc 类似物的合成及其作为几丁质合酶底物的评估。
DOI：
10.1021/jo0483670
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Finney,NathanielS
中科院分区：
文献类型：
--
作者：
Yeager,AdamR;Finney,NathanielS
几丁质合成酶（Chitin synthase，CS）是一种将UDP-GlcNAc聚合形成几丁质（poly-β（1，4）-GlcNAc）的酶，是真菌细胞壁生物合成的关键组分。关于几丁质合成酶的底物特异性或酶将耐受的底物修饰的范围知之甚少。继以前的报告表明，6-O-丹磺酰基GlcNAc生物合成纳入甲壳素，我们开始有兴趣开发一种测定CS活性的基础上掺入的荧光底物。我们描述了两个荧光UDP-GlcNAc类似物的合成及其作为几丁质合成酶底物的评价。
Chitin synthase (CS) polymerizes UDP-GlcNAc to form chitin (poly-β(1,4)-GlcNAc), a key component of fungal cell wall biosynthesis. Little is known about the substrate specificity of chitin synthase or the scope of substrate modification the enzyme will tolerate. Following a previous report suggesting that 6-O-dansyl GlcNAc is biosynthetically incorporated into chitin, we became interested in developing an assay for CS activity based on incorporation of a fluorescent substrate. We describe the synthesis of two fluorescent UDP-GlcNAc analogues and their evaluation as chitin synthase substrates.