Frequent Paternal Mitochondrial Inheritance and Rapid Haplotype Frequency Shifts in Copepod Hybrids
Frequent Paternal Mitochondrial Inheritance and Rapid Haplotype Frequency Shifts in Copepod Hybrids
复制标题
桡足类杂种中频繁的父系线粒体遗传和快速单倍型频率变化
DOI：
10.1093/jhered/esab068
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发表时间：
2021
影响因子：
3.1
通讯作者：
Willett, Christopher S
中科院分区：
文献类型：
--
作者：
Lee, Jeeyun;Willett, Christopher S
线粒体在大多数动物物种中被认为是母系遗传的，这一基本概念促进了系统发育、保护和种群遗传学的进步。和其他动物一样，线粒体被认为是海洋桡足类老虎的唯一母系遗传，这已经成为研究有丝分裂相互作用、杂交破裂和环境耐受性的有用模型。然而，我们提出了PCR、Sanger测序和Illumina NextEra测序的证据，证明父本线粒体DNA(MtDNA)在种群间杂交中发生了广泛的传播。卡福尼克斯。在3个群体(总样本量为376个F1个体)间具有20%全基因组线粒体差异的4种杂交组合上，发现2%~59%的F1同时含有父本和母本mtDNA，在同一群体对的不同杂交方向上，父本漏值分别为低和高。测序方法进一步验证了F1 mtDNA和父本mtDNA序列之间的核苷酸相似性。有趣的是，一些杂交F1的父本mtDNA遗传了在父本群体中不常见的单倍型。与以往一些关于父本线粒体DNA泄漏的研究相比，我们采用了更严格的方法来排除由于无功能的核线粒体DNA片段对父亲mtDNA的污染和误检。我们的结果提出了这样一种可能性，即被认为只遗传母体线粒体的其他动物系统也可能存在父系泄漏，这将影响对过去和未来种群遗传学或依赖线粒体作为单亲标记的系统发育研究的解释。
Mitochondria are assumed to be maternally inherited in most animal species, and this foundational concept has fostered advances in phylogenetics, conservation, and population genetics. Like other animals, mitochondria were thought to be solely maternally inherited in the marine copepodTigriopus californicus,which has served as a useful model for studying mitonuclear interactions, hybrid breakdown, and environmental tolerance. However, we present PCR, Sanger sequencing, and Illumina Nextera sequencing evidence that extensive paternal mitochondrial DNA (mtDNA) transmission is occurring in inter-population hybrids ofT. californicus. PCR on four types of crosses between three populations (total sample size of 376 F1 individuals) with 20% genome-wide mitochondrial divergence showed 2% to 59% of F1 hybrids with both paternal and maternal mtDNA, where low and high paternal leakage values were found in different cross directions of the same population pairs. Sequencing methods further verified nucleotide similarities between F1 mtDNA and paternal mtDNA sequences. Interestingly, the paternal mtDNA in F1s from some crosses inherited haplotypes that were uncommon in the paternal population. Compared to some previous research on paternal leakage, we employed more rigorous methods to rule out contamination and false detection of paternal mtDNA due to non-functional nuclear mitochondrial DNA fragments. Our results raise the potential that other animal systems thought to only inherit maternal mitochondria may also have paternal leakage, which would then affect the interpretation of past and future population genetics or phylogenetic studies that rely on mitochondria as uniparental markers.