Enhanced photostability of an anthracene-based dye due to supramolecular encapsulation: A new type of photostable fluorophore for single-molecule study
Enhanced photostability of an anthracene-based dye due to supramolecular encapsulation: A new type of photostable fluorophore for single-molecule study
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超分子封装增强蒽基染料的光稳定性：用于单分子研究的新型光稳定荧光团
DOI：
10.1039/c4pp00065j
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
K. Kobayashi
中科院分区：
文献类型：
--
作者：
M. Mitsui;K. Higashi;R. Takahashi;Y. Hirumi;K. Kobayashi
对于单分子荧光研究，高度光稳定的荧光团是绝对必要的，因为光诱导降解（即，光漂白）限制了单个分子的观察时间。本文采用单分子荧光光谱法（SMFS）研究了一种高荧光的9，10-双（苯乙炔基）蒽衍生物（G）及其自组装硼酸酯包封复合物（G@Cap）在玻璃态聚合物基质中的光物理性质和光稳定性。的异质性的荧光发射波长和三重闪烁动力学的客户G显着降低超分子封装由于构象的限制和减少的异质性，在当地的环境。通过评估G和G@Cap的光漂白产率，定量证实了由于包封导致的G的光稳定性增加近10倍。此外，发现G@Cap的光稳定性比罗丹明6 G（一种在单分子研究中广泛使用的荧光染料）高>30倍。这些结果表明，G@Cap可以作为一个非常明亮，持久的荧光探针的单分子研究。
For single-molecule fluorescence studies, highly photostable fluorophores are absolutely imperative, because photo-induced degradation (i.e., photobleaching) limits the observation time of individual molecules. Herein, the photophysics and photostability of a highly fluorescent 9,10-bis(phenylethynyl)anthracene derivative (G) and its self-assembled boronic ester encapsulation complex (G@Cap) embedded in a glassy polymer matrix are investigated by single-molecule fluorescence spectroscopy (SMFS). The heterogeneity of the fluorescence emission wavelength and triplet blinking kinetics of the guest G are significantly decreased by supramolecular encapsulation due to conformational restriction and reduced heterogeneity in the local environment. A nearly 10-fold increase in the photostability of G due to encapsulation is quantitatively confirmed by evaluating the photobleaching yields of G and G@Cap. In addition, it is found that the G@Cap is >30-fold more photostable than rhodamine 6G, a widely used fluorescent dye in single-molecule studies. These results demonstrate that the G@Cap can serve as a very bright, long-lasting fluorescent probe for single-molecule studies.