ISOLATION OF A CDNA CLONE FOR CHICK INTESTINAL APOLIPOPROTEIN-A-I (APO-AI) AND ITS USE FOR DETECTING APO-AI MESSENGER-RNA EXPRESSION IN SEVERAL CHICK-TISSUES
ISOLATION OF A CDNA CLONE FOR CHICK INTESTINAL APOLIPOPROTEIN-A-I (APO-AI) AND ITS USE FOR DETECTING APO-AI MESSENGER-RNA EXPRESSION IN SEVERAL CHICK-TISSUES
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DOI：
10.1016/0378-1119(86)90298-2
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发表时间：
1986-01-01
期刊：
影响因子：
3.5
通讯作者：
TARUGI, P
中科院分区：
文献类型：
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作者：
FERRARI, S;DRUSIANI, E;TARUGI, P
鸡载脂蛋白AI（Apo-AI）是血浆高密度脂蛋白的主要蛋白组分，已分离出三个cDNA克隆。首先通过用抗Apo-AI抗体筛选pEX 1表达载体中的cDNA文库，其次通过对阳性克隆表达的蛋白质进行Western印迹分析，确定了克隆的身份。在分子杂交实验中使用的克隆含有最大的，大概是全尺寸的cDNA插入片段（apo 5C 12）证实，apo-AI mRNA主要在鸡小肠和肝脏中表达。此外，我们提供的证据表明，大脑，心脏和骨骼肌也合成大量的apo-AI mRNA。限制性内切酶消化的鸡DNA与apo 5C 12 DNA的Southern印迹杂交模式与apo-AI基因的单拷贝一致。
Three cDNA clones for chick apolipoprotein AI(Apo-AI), the major protein component of plasma high-density lipoproteins, have been isolated. The identity of the clones has been established first by screening a cDNA library in the pEX1 expression vector with anti-Apo-AI antibodies, second by Western blot analysis of the proteins expressed by positive clones. The use of the clone containing the largest, presumably full-size, cDNA insert (apo5C12) in molecular hybridization experiments confirms that apo-AI mRNA is expressed mainly in chick small intestine and liver. Furthermore, we provide evidence that brain, heart and skeletal muscle also synthesize significant amounts of apo-AI mRNA. The Southern-blot hybridization pattern of the restriction-enzyme-digested chick DNA with the apo5C12 DNA is consistent with there being a single copy of the apo-AI gene.