Identification of components in Zhi-Zi-Da-Huang decoction by HPLC coupled with electrospray ionization tandem mass spectrometry, photodiode array and fluorescence detectors
Identification of components in Zhi-Zi-Da-Huang decoction by HPLC coupled with electrospray ionization tandem mass spectrometry, photodiode array and fluorescence detectors
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DOI：
10.1016/j.jpba.2009.02.023
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发表时间：
2009-07-12
影响因子：
3.4
通讯作者：
Feng, Fang
中科院分区：
文献类型：
--
作者：
Wang, Hang;Feng, Fang
采用高效液相色谱法（HPLC）、电喷雾离子（ESI）串联质谱法（MS）、光电二极管阵列（PDA）和荧光检测器（FD）同时对栀子大黄汤（ZZDHD）中的30种有效成分进行了表征。分析在Lichrospher C-18色谱柱（4.6 mm × 250 mm, 5 μ m）上进行，使用二元洗脱液(0.1%醋酸水溶液（a)和甲醇(B), 1 ml/min）在梯度条件下进行。根据保留时间（t(R)）、紫外光谱数据、荧光特性、提取离子电流（EIC）结果、分子量和质量碎片行为信息。17类黄酮。检出5种蒽醌类、2种香豆素类和6种环烯醚萜。并对环烯醚萜类、黄酮类、蒽醌类、香豆素类进行了ESI-MS/MS分析。提出的破碎模式可以扩展到上述成分，但在不同的草药和方剂中。该方法适用于草药体系和其他复杂样品中未知成分的鉴别，特别是在没有标准品的情况下。(C) 2009 Elsevier B.V.版权所有
High-performance liquid chromatography (HPLC) coupled with electrospray ionization (ESI) tandem mass spectrometry (MS) in positive and negative ion mode and photodiode array (PDA) and fluorescence detectors (FD) was applied to simultaneously characterise thirty active components in Zhi-Zi-Da-Huang decoction (ZZDHD). The analysis was preformed on a Lichrospher C-18 column (4.6 mm x 250 mm, 5 mu m) using a binary eluent (0.1% aqueous acetic acid (A) and methanol (B), I ml/min) under gradient conditions. Based on the retention times (t(R)), UV spectral data, fluorescence characteristics, results of extracted ion current (EIC), information of molecular weight and mass fragmentation behaviours. 17 flavonoids. five anthraquinones, two coumarins and six iridoids were detected and identified. The ESI-MS/MS fragmentation behaviour of iridoids, flavonoids, anthraquinones, coumarins was also proposed. The fragmentation patterns proposed could be extended to the components mentioned above but in different herbs and prescriptions. The method was useful for the identification of unknown components in the herbal system and other complex samples, especially for which standards are unavailable. (C) 2009 Elsevier B.V. All rights reserved.