Biological Effects of the Herbal Plant-Derived Phytoestrogen Bavachin in Primary Rat Chondrocytes.
Biological Effects of the Herbal Plant-Derived Phytoestrogen Bavachin in Primary Rat Chondrocytes.
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DOI：
10.1248/bpb.b15-00198
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发表时间：
2015-08
影响因子：
2
通讯作者：
Gyeong-Je Lee;In-A Cho;Kyeong-Rok Kang;Do Kyung Kim;H. Sohn;J. You;Ji-Su Oh;Yo-Seob Seo;
中科院分区：
文献类型：
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作者：
Gyeong-Je Lee;In-A Cho;Kyeong-Rok Kang;Do Kyung Kim;H. Sohn;J. You;Ji-Su Oh;Yo-Seob Seo;
本研究的目的是检查补骨脂黄素在原代大鼠软骨细胞中的合成代谢和抗分解代谢功能。 Bavachin处理后，软骨细胞存活21 d，无细胞增殖，且蛋白多糖含量和细胞外基质增加。软骨细胞的短期单层培养表明，骨蛋白聚糖和 II 型胶原蛋白（主要的细胞外基质成分）的基因诱导均被 Bavachin 显着上调。 Bavachin 显着抑制软骨降解酶（例如基质金属蛋白酶、解整合素和具有血小板反应蛋白基序的金属蛋白酶）的表达和活性，而金属蛋白酶的组织抑制剂显着上调。 Bavachin 抑制软骨细胞中代表性分解代谢因子诱导型一氧化氮合酶的表达，并以剂量依赖性方式下调一氧化氮、环氧合酶-2 和前列腺素 E2 的表达。我们的研究结果表明，巴瓦钦对软骨细胞具有合成代谢和有效的抗分解代谢生物学作用，这在未来治疗关节软骨退化方面可能具有相当大的前景。
The aim of this study was to examine the anabolic and anticatabolic functions of bavachin in primary rat chondrocytes. With bavachin treatment, chondrocytes survived for 21 d without cell proliferation, and the proteoglycan content and extracellular matrix increased. Short-term monolayer culture of chondrocytes showed that gene induction of both aggrecan and collagen type II, major extracellular matrix components, was significantly upregulated by bavachin. The expression and activities of cartilage-degrading enzymes such as matrix metalloproteinases and a disintegrin and metalloproteinase with thrombospondin motifs were inhibited significantly by bavachin, while tissue inhibitors of metalloprotease were significantly upregulated. Bavachin inhibits the expression of inducible nitric oxide synthase, a representative catabolic factor, and downregulated the expression of nitric oxide, cyclooxygenase-2, and prostaglandin E2 in a dose-dependent manner in chondrocytes. Our results suggest that the bavachin has anabolic and potent anticatabolic biological effects on chondrocytes, which may have considerable promise in treating articular cartilage degeneration in the future.