In Vivo Antitumor and Antioxidative Effects of a Rapeseed Meal Protein Hydrolysate on an S180 Tumor-Bearing Murine Model
In Vivo Antitumor and Antioxidative Effects of a Rapeseed Meal Protein Hydrolysate on an S180 Tumor-Bearing Murine Model
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DOI：
10.1271/bbb.90374
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发表时间：
2009-11
期刊：
影响因子：
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通讯作者：
Zhaohui Xue;Wancong Yu;Moucheng Wu;Jiehua Wang
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文献类型：
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作者：
Zhaohui Xue;Wancong Yu;Moucheng Wu;Jiehua Wang
采用昆明小鼠体内S180荷瘤模型，评价了从菜籽粕中提取的菜籽蛋白水解物（RSCH）的抗肿瘤和抗氧化活性。荷瘤雌性小鼠经胃灌注给予0、50、100和150 mg/kg/d剂量的RSCH，持续10天。在100和150 mg/kg/d剂量组中，RSCH分别使肿瘤重量显著降低44%和53%，而未导致死亡或生长迟缓。在150 mg/kg/d组中，胸腺和脾脏指数（器官重量相对于体重）显著增加。RSCH 150 mg/kg/d剂量组小鼠腹腔巨噬细胞吞噬能力和迟发型超敏反应（DTH）显著增强。RSCH给药还增强了超氧化物歧化酶活性，降低了血清中硫代巴比妥酸反应物质的水平。我们的研究结果表明，口服RSCH给药具有抗肿瘤保护作用，并可能通过减少小鼠模型中的自由基形成和氧化应激来改善免疫功能。
The antitumor and antioxidative activities of a rapeseed protein hydrolysate (RSCH) obtained from rapeseed meal were evaluated by using an in vivo S180 tumor-bearing Kunming mice model. Tumor-bearing female mice were given RSCH for 10 at doses of 0, 50, 100, and 150 mg/kg/d by gastric perfusion. RSCH significantly decreased the tumor weight by 44% and 53% in the 100 and 150 mg/kg/d groups, respectively, without causing mortality or growth retardation. The thymus and spleen indices (organ weight relative to body weight) were increased significantly in the 150 mg/kg/d group. The phagocytic capability of coeliac macrophages and delayed-type hypersensitivity (DTH) were significantly increased in tumor-bearing mice treated with RSCH at 150 mg/kg/d. RSCH administration also enhanced the superoxide dismutase activity and reduced the serum level of thiobarbituric acid reactive substances. Our results show that an oral RSCH administration had an antitumor protective effect and may improve immune function by reducing free radical formation and oxidative stress in a murine model.