Exchangeable immobilized enzyme reactor for enzyme inhibition tests in flow-injection analysis using a magnetic device. Determination of pesticides in drinking water

Exchangeable immobilized enzyme reactor for enzyme inhibition tests in flow-injection analysis using a magnetic device. Determination of pesticides in drinking water
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DOI:
10.1016/s0003-2670(00)83545-x
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发表时间:
1990
影响因子:
6.2
通讯作者:
R. Kindervater;W. Künnecke;R. Schmid
R. Kindervater;W. Künnecke;R. Schmid
中科院分区:
化学1区
文献类型:
--
作者:
R. Kindervater;W. Künnecke;R. Schmid

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A flow-injection system for rapid automated enzyme inhibition testing is described. Exchange of inactivated enzyme immobilized on magnetic particles was performed with magnetic devices which could be electrically switched off to release all bound material. The flow resistance of the reactor was excellent. Inhibition of immobilized acetylcholinesterase was used to determine pesticides in drinking water. Concentrations of 0.5 μg l−1of the pesticides carbofuran and malaoxon were detected. A complete cycle of analysis, including calibration, took 20 min.