Effect of interleukin 2, interferon-gamma, and mitogens on the production of tumor necrosis factors alpha and beta.
Effect of interleukin 2, interferon-gamma, and mitogens on the production of tumor necrosis factors alpha and beta.
复制标题
白细胞介素 2、干扰素-γ 和有丝分裂原对肿瘤坏死因子 α 和 β 产生的影响。
DOI：
10.4049/jimmunol.135.4.2492
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发表时间：
1985
影响因子：
4.4
通讯作者：
D. Goeddel
中科院分区：
文献类型：
--
作者：
G. Nedwin;L. P. Svedersky;T. Bringman;M. Palladino;D. Goeddel
人外周血单个核细胞（PBMC）被重组白细胞介素2和有丝分裂原诱导分泌两种不同的细胞毒性多肽，肿瘤坏死因子-α（TNF-α）和肿瘤坏死因子-β（TNF-β），以前被称为α光毒素。用重组人白细胞介素2（rIL 2）或促有丝分裂剂与重组人干扰素-γ（rIFN-γ）组合治疗PBMC导致TNF-α和TNF-β的产生增加。单独的rIFN-γ对任一细胞毒性多肽的产生没有影响。TNF-α在诱导后2至3小时内产生，并且是PBMC在培养的前48小时期间产生的主要细胞毒素，此后TNF-β成为主要种类。在诱导8小时后，TNF-β首先分泌到培养基中。当PBMC被分离成贴壁和非贴壁细胞时，观察到TNF-α和TNF-β的水平增强。在造血来源的不同肿瘤细胞系中诱导TNF-α和TNF-β。结果表明，TNF-α和TNF-β的产生可以被两种淋巴因子IL-2和IFN-γ增强。
Human peripheral blood mononuclear cells (PBMC) were induced by recombinant interleukin 2 and mitogens to secrete two distinct cytotoxic polypeptides, tumor necrosis factor-alpha (TNF-alpha) and tumor necrosis factor-beta (TNF-beta), previously called lymphotoxin. Treatment of PBMC with recombinant human interleukin 2 (rIL 2) or mitogens in combination with recombinant human interferon-gamma (rIFN-gamma) resulted in augmented production of both TNF-alpha and TNF-beta. rIFN-gamma alone had no effect on production of either cytotoxic polypeptide. TNF-alpha was produced within 2 to 3 hr after induction and was the major cytotoxin produced by PBMC during the first 48 hr of culture, after which time TNF-beta became the predominant species. TNF-beta was first secreted into the media after 8 hr of induction. Enhanced levels of both TNF-alpha and TNF-beta were seen when the PBMC were separated into adherent and nonadherent cells. Both TNF-alpha and TNF-beta were induced in different tumor cell lines of hematopoietic origin. The results demonstrate that the production of TNF-alpha and TNF-beta can be enhanced by two lymphokines, IL 2 and IFN-gamma.