Proteomic characterization of rat liver exposed to 2,3,7,8-tetrachlorobenzo-p-dioxin
Proteomic characterization of rat liver exposed to 2,3,7,8-tetrachlorobenzo-p-dioxin
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DOI：
10.1021/pr049830s
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发表时间：
2005-03-01
影响因子：
4.4
通讯作者：
Kim, CW
中科院分区：
文献类型：
--
作者：
Lee, SH;Lee, DY;Kim, CW
二恶英是一类多卤代芳烃，可在实验动物中引起广泛的毒性反应。在这项研究中，将2，3，7，8-四氯苯并对二恶英（TCDD）暴露于两组SD大鼠中;一组以1、10、20和50 μ g/kg体重的单次剂量进行短期暴露（第1组），另一组以0.01、0.1、1和2.5 μ g/kg体重的每日低剂量进行长期暴露（第2组）一个月。采用双向电泳技术对不同剂量TCDD染毒大鼠肝脏蛋白质组进行分析。在2-DE分析中，ESI-Q-TOF MS/MS检测到2个新表达的斑点和7个体积增加的斑点，特别是蛋白酶体β亚基3在所有剂量下均增加。此外，在第2组中，筛选出6个体积增加的斑点;特别是，组氨酸三联体核苷酸结合蛋白在0.1 μ g/kg剂量和1 μ g/kg剂量下均增加。Western blot鉴定蛋白质。其中载脂蛋白A-IV可能对TCDD暴露诱导的脂质过氧化和动脉粥样硬化具有保护作用，而磷酸甘油酸酯β 1的表达水平则因TCDD暴露诱导的甲状腺功能亢进而升高。
Dioxins are a class of polyhalogenated aromatic hydrocarbons that induce a wide spectrum of toxic responses in experimental animals. In this study, 2,3,7,8-tetrachlorobenzo-p-dioxin (TCDD) was exposed to two SD rat groups; one group for short-term exposure at a single dose of 1, 10, 20 and 50 mu g/kg body weight (group 1) and the other for long-term exposure at daily and-low dose of 0.01, 0.1, 1 and 2.5 mu g/kg body weight (group 2) for a month. Two-dimensional electrophoresis (2-DE) was utilized to resolve the protein profile of rat liver exposed to TCDD at different doses. In the analysis of 2-DE of the group 1, two new-expressed spots and seven volume-increased spots were detected and identified by ESI-Q-TOF MS/MS; especially, proteasome subunit beta type 3 was increased in all doses. In addition, in the group 2, six volume-increased spots were screened; particularly, histidine triad nucleotide binding protein was increased in both 0.1 mu g/kg dose and 1 mu g/kg dose. The identified proteins were confirmed using Western blot. Among the identified proteins, apolipoprotein A-IV may protect lipid peroxidation and atherosclerosis induced by TCDD exposure and the expression level of phosphoglycerate mutase increases due to hyperthyroidism induced by TCDD exposure.