Peripheral Nerve in a Novel Rat Model of
Peripheral Nerve in a Novel Rat Model of
复制标题
新型大鼠模型中的周围神经
DOI：
10.1007/978-1-0716-0655-1_9
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Hibi Hideharu
中科院分区：
文献类型：
--
作者：
Sakai Kiyoshi;Tsuruta Takeshi;Watanabe Junna;Sugimura Yukiko;Sakaguchi Kohei;Katagiri Wataru;Hibi Hideharu
上级喉神经（SLN）在喉反射和吞咽中起重要作用。SLN的损伤导致吞咽困难，即吞咽困难。我们成功地建立了一种新的大鼠SLN损伤性吞咽障碍模型，在该模型中，我们可以评估来自人脱落乳牙的干细胞（SHED）的神经再生能力。吞咽困难的大鼠表现出体重减轻和饮酒模式改变。此外，SLN损伤诱导吞咽反射的延迟发作和喉部碎片在咽中的积累。该大鼠模型用于评价SHED条件培养基（SHED-CM）作为吞咽困难治疗候选物的全身应用。我们发现，SHED-CM促进SLN的功能恢复和显着的轴突再生，通过极化的巨噬细胞从活化的炎症巨噬细胞（M1）到抗炎巨噬细胞（M2）和血管生成。本章介绍了SLN损伤致吞咽困难大鼠模型的建立及SHED-CM的制备和应用。
The superior laryngeal nerve (SLN) is known to play an essential role in the laryngeal reflex and swallowing. Damage to the SLN causes difficulty swallowing, that is, dysphagia. We successfully developed a novel rat model of dysphagia by SLN injury, in which we could evaluate the neuroregenerative capacity of stem cell from human exfoliated deciduous teeth (SHED). The dysphagic rats exhibit weight loss and altered drinking patterns. Furthermore, SLN injury induces a delayed onset of the swallowing reflex and accumulation of laryngeal debris in the pharynx. This rat model was used to evaluate the systemic application of SHED-conditioned medium (SHED-CM) as a therapeutic candidate for dysphagia. We found that SHED-CM promoted functional recovery and significant axonal regeneration in SLNs through the polarization shift of macrophages from activated inflammatory macrophages (M1) to anti-inflammatory macrophages (M2) and angiogenesis. This chapter describes the establishment of SLN-injury induced dysphagia rat model and the preparation and application of SHED-CM.