Group II subfamily secretory phospholipase A2 enzymes:: expression in chronic rhinosinusitis with and without nasal polyps
Group II subfamily secretory phospholipase A2 enzymes:: expression in chronic rhinosinusitis with and without nasal polyps
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DOI：
10.1111/j.1398-9995.2007.01381.x
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发表时间：
2007-09-01
期刊：
影响因子：
12.4
通讯作者：
Cui, Y. H.
中科院分区：
文献类型：
--
作者：
Liu, Z.;Lu, X.;Cui, Y. H.
背景：Ⅱ类分泌型磷脂酶A（2）（sPLA（2）s）是一类在炎症反应中起重要作用的酶。然而，在人类鼻窦粘膜中存在的第II组亚家族sPLA（2）s及其在慢性鼻窦炎（CRS）中的作用尚不清楚。本研究的目的是研究第II组亚家族sPLA（2）s在正常人和CRS患者鼻息肉（NPs）和非鼻息肉（NPs）的人鼻窦粘膜中的表达以及促炎细胞因子对表达的调节。通过逆转录聚合酶链反应（RT-PCR）研究手术样本，以评估第II组亚家族sPLA（2）s mRNA的表达，用免疫组化方法分析Ⅱ型亚家族sPLA（2）s阳性细胞的存在和位置。结果：与对照组相比，CRS患者组织中sPLA（2）-IIA、-IID和-IIE的mRNA表达显著上调，而对照组中sPLA（2）-IIA、-IID和-IIE的mRNA表达显著上调。在CRS患者中，无NP的患者的鼻窦粘膜中sPLA（2）-IIA mRNA的表达显著高于NP的患者，而sPLA（2）-IIE mRNA的表达较弱。免疫组织化学显示CRS患者样本中上皮细胞和粘膜下腺体中II型sPLA（2）和特异性IIA型sPLA（2）的蛋白表达增强。与NP相比，在来自没有NP的CRS患者的样本中发现了更强的IIA型sPLA（2）蛋白表达。结论：CRS组织中sPLA（2）s Ⅱ类亚家族部分成员表达上调，可能与IL-1 β和TNF-α的过度表达有关。不同的第II组亚家族sPLA（2）s可能在有和没有NP的CRS发病机制中发挥不同的作用。
Background: Group II subfamily secretory phospholipases A(2) (sPLA(2)s) are the enzymes that can play a major role in inflammation. However, the presence of group II subfamily sPLA(2)s in human sinonasal mucosa and their roles in chronic rhinosinusitis (CRS) are not well known. The purpose of this study was to investigate the expression of group II subfamily sPLA(2)s in human sinonasal mucosa from controls and CRS patients with and without nasal polyps (NPs) and the regulation of expression by proinflammatory cytokines.Methods: Surgical samples were investigated by means of reverse transcriptase polymerase chain reaction (RT-PCR) for evaluation of group II subfamily sPLA(2)s mRNA expression, and the presence and location of group II subfamily sPLA(2)s-positive cells were analyzed by means of immunohistochemistry. Furthermore, nasal explant culture and quantitative RT-PCR techniques were used to investigate the effect of interleukin (IL)-1 beta and tumor necrosis factor (TNF)-alpha on group II subfamily sPLA(2)s mRNA production in sinonasal mucosa.Results: Messenger RNA expression of sPLA(2)-IIA, -IID, and -IIE was significantly upregulated in tissues from CRS patients compared with control tissues. Among CRS patients, patients without NPs showed significantly stronger expression in sinonasal mucosa than patients with NPs of sPLA(2)-IIA mRNA, and weaker expression of sPLA(2)-IIE mRNA. Immunohistochemistry revealed enhanced protein expression of type II sPLA(2)s and specific type IIA sPLA(2) in epithelial cells and submucosal glands in samples from CRS patients. Stronger type IIA sPLA(2) protein expression was found in samples from CRS patients without NPs when compared with NPs. Nasal explant culture experiments demonstrated that mRNA expression of sPLA(2)-IIA, -IID, and -IIE was dramatically induced by IL-1 beta and TNF-alpha.Conclusions: The expression of some members of group II subfamily of sPLA(2)s is upregulated in CRS and it may result from IL-1 beta and TNF-alpha overexpression. Different individual group II subfamily sPLA(2)s may play different roles in the pathogenesis of CRS with and without NPs.