Anther developmental defects in Arabidopsis thaliana male-sterile mutants
Anther developmental defects in Arabidopsis thaliana male-sterile mutants
复制标题
DOI：
10.1007/s004970050158
复制
发表时间：
1999-01-01
影响因子：
--
通讯作者：
Goldberg, RB
中科院分区：
文献类型：
--
作者：
Sanders, PM;Bui, AQ;Goldberg, RB
我们通过筛选T-DNA和ems诱变系鉴定了拟南芥不育突变体，并鉴定了几种具有不同花药过程特异性缺陷的雄性不育突变体。从T-DNA和EMS筛选中分别发现了大约44和855个不育突变体。详细研究了几种突变体的缺陷，包括花药形态的建立、小孢子的产生、花粉分化和花药开裂。发现了不开裂和晚开裂突变体。此外，无花粉突变体在小室周围的细胞层中观察到明显的减数分裂缺陷和/或异常。在POLLENLESS3位点上发现了两个突变等位基因，它们在产生功能性小孢子方面存在缺陷，导致花药室内细胞退化。无花粉3-1含有一个与突变表型共分离的T-DNA插入，无花粉3-2在花粉3基因中有一个大的缺失。POLLENLESS3基因在基因库中没有已知的对应基因，但它编码一种含有假定的核定位和蛋白-蛋白相互作用基序的蛋白质。POLLENLESS3基因最近被证明与MS5相同，MS5是先前描述的拟南芥雄性不育突变体。在POLLENLESS3基因组区鉴定出3个基因：GENEY、POLLENLESS3和β 9 TUBULIN。拟南芥基因组中含有POLLENLESS3和β 9-TUBULIN基因的片段被复制并存在于不同的染色体上。对POLLENLESS3表达模式的分析表明，1.3 kb的POLLENLESS3 mRNA特异性定位于花药室的减数分裂细胞中，并且仅在减数分裂后期存在。
We identified Arabidopsis thaliana sterility mutants by screening T-DNA and EMS-mutagenized lines and characterized several male-sterile mutants with defects specific for different anther processes. Approximately 44 and 855 sterile mutants were uncovered from the T-DNA and EMS screens, respectively. Several mutants were studied in detail with defects that included the establishment of anther morphology, microspore production, pollen differentiation, and anther dehiscence. Both non-dehiscencing and late-dehiscencing mutants were identified. In addition, pollenless mutants were observed with either apparent meiotic defects and/or abnormalities in cell layers surrounding the locules. Two mutant alleles were identified for the POLLENLESS3 locus which have defects in functional microspore production that lead to the degeneration of cells within the anther locules. pollenless3-1 contains a T-DNA insertion that co-segregates with the mutant phenotype and pollenless3-2 has a large deletion in the POLLENLESS3 gene. The POLLENLESS3 gene has no known counterparts in the GenBank, but encodes a protein containing putative nuclear localization and protein-protein interaction motifs. The POLLENLESS3 gene was shown recently to be the same as MS5, a previously described Arabidopsis thaliana male-sterility mutant. Three genes were identified in the POLLENLESS3 genomic region: GENEY, POLLENLESS3, and beta 9 TUBULIN. The segment of the Arabidopsis thaliana genome containing the POLLENLESS3 and beta 9-TUBULIN genes is duplicated and present on a different chromosome. Analysis of the POLLENLESS3 expression pattern determined that the 1.3-kb POLLENLESS3 mRNA is localized specifically within meiotic cells in the anther locules and that POLLENLESS3 mRNA is present only during late meiosis.