A new, easy-to-make pectin-honey hydrogel enhances wound healing in rats.
A new, easy-to-make pectin-honey hydrogel enhances wound healing in rats.
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DOI：
10.1186/s12906-017-1769-1
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发表时间：
2017-05-16
影响因子：
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通讯作者：
Gandini M
中科院分区：
文献类型：
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作者：
Giusto G;Vercelli C;Comino F;Caramello V;Tursi M;Gandini M
蜂蜜，单独或组合，自古以来就被用于伤口愈合，并在过去十年中重新成为人们感兴趣的话题。果胶最近被研究用于各种生物医学应用，如药物递送、皮肤保护和细胞支架。本研究的目的是开发和评价果胶-蜂蜜水凝胶（PHH）作为伤口愈合膜，并将该敷料与液体蜂蜜进行比较。将36只成年雄性Sprague-Dawley大鼠麻醉，并在背部创建一个2 × 2 cm的切除伤口。将动物随机分配到四个组（PHH、LH、Pec和C）：在PHH组中，将果胶-蜂蜜水凝胶应用于伤口上的绷带下;在LH组中，应用液体麦卢卡蜂蜜;在Pec组中，应用果胶水凝胶（Pec）;并且在C组中，仅将绷带应用于伤口。在规定的时间点拍摄伤口图像，计算伤口面积缩小率并进行组间比较。与对照组相比，PHH、LH和Pec组的伤口面积缩小速度更快，PHH组的速度明显更快。令人惊讶的是，Pec组比LH组表现出更快的伤口愈合，但这种效果在统计学上并不显著。这是第一项使用果胶与蜂蜜结合生产用于伤口治疗的生物医学水凝胶的研究。结果表明，PHH的使用对于促进和加速伤口愈合是有效的。
Honey, alone or in combination, has been used for wound healing since ancient times and has reemerged as a topic of interest in the last decade. Pectin has recently been investigated for its use in various biomedical applications such as drug delivery, skin protection, and scaffolding for cells. The aim of the present study was to develop and evaluate a pectin-honey hydrogel (PHH) as a wound healing membrane and to compare this dressing to liquid honey. Thirty-six adult male Sprague-Dawley rats were anesthetized and a 2 × 2 cm excisional wound was created on the dorsum. Animals were randomly assigned to four groups (PHH, LH, Pec, and C): in the PHH group, the pectin-honey hydrogel was applied under a bandage on the wound; in the LH group, liquid Manuka honey was applied; in the Pec group, pectin hydrogel was applied (Pec); and in the C group, only bandage was applied to the wound. Images of the wound were taken at defined time points, and the wound area reduction rate was calculated and compared between groups. The wound area reduction rate was faster in the PHH, LH, and Pec groups compared to the control group and was significantly faster in the PHH group. Surprisingly, the Pec group exhibited faster wound healing than the LH group, but this effect was not statistically significant. This is the first study using pectin in combination with honey to produce biomedical hydrogels for wound treatment. The results indicate that the use of PHH is effective for promoting and accelerating wound healing.