Tissue Culture Responsive MicroRNAs in Strawberry
Tissue Culture Responsive MicroRNAs in Strawberry
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草莓中的组织培养响应性 MicroRNA
DOI：
10.1007/s11105-011-0406-2
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发表时间：
2012-08-01
影响因子：
2.1
通讯作者：
Zhang, Zhihong
中科院分区：
文献类型：
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作者：
Li, He;Zhao, Xiaochu;Zhang, Zhihong
MicroRNAs（miRNAs）是一类长度为20-24个核苷酸的非编码调控RNA，在植物生长发育中起着重要作用。由RNA聚合酶II转录的miRNA编码基因参与多种过程，包括发育和形态发生系统以及激素和应激反应。为了研究miRNA对组织培养条件的响应，使用定量逆转录-聚合酶链反应（qRT-PCR）来检测体外微繁殖草莓植物和移植微繁殖草莓植物之间miRNA表达的差异。结果显示，在正常对照组和正常对照组中，有4个miRNAs表达差异，其中1个miR 156表达上调，3个miR 164、miR 172和miR 390表达下调。在离体微繁殖草莓植株中，miR 156、miR 164、miR 172和miR 390的miRNA表达量与温室土壤中移植4个月的草莓植株的miRNA表达量之比分别为6.757、0.046、0.035和0.050。移植到土壤中5个月的微繁殖植物中的miR 156表达水平与常规繁殖的亚军植物中的水平的比率为3.785。miR 156在草莓离体微繁殖植株中高表达，且与其靶基因SPL 9和miR 172的表达量成显著的反比。我们推测miR 156的高表达是微繁殖植物复壮的主要原因。
MicroRNAs (miRNAs) are 20-24 nucleotide (nt) non-coding regulatory RNAs which play critical roles in plant growth and development. miRNA-encoding genes, which are transcribed by RNA polymerase II, are involved in a variety of processes, including developmental and morphogenesis systems, and hormone and stress responses. To investigate miRNA responses to tissue culture conditions, quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to detect differences in miRNA expression between in vitro micropropagated strawberry plants and transplanted micropropagated strawberry plants. Four miRNAs were differentially expressed between them, including one up-regulated gene (miR156) and three down-regulated genes (miR164, miR172 and miR390). The ratios of miRNA expression levels in in vitro micropropagated strawberry plants to micropropagated plants transplanted into soil in greenhouse for 4 months for miR156, miR164, miR172 and miR390 were 6.757, 0.046, 0.035 and 0.050, respectively. The ratio of miR156 expression levels in micropropagated plants transplanted into soil for 5 months to levels in the conventionally propagating runner plants was 3.785. miR156 was expressed highly and was strikingly inversely proportional to the expressions of its target gene SPL9 and miR172 in in vitro micropropagated strawberry plants. We speculate that high expression of miR156 is the main reason for rejuvenation in micropropagated plants.