Differential expression of human major histocompatibility class I loci: HLA-A, -B, and -C
Differential expression of human major histocompatibility class I loci: HLA-A, -B, and -C
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DOI：
10.1016/s0198-8859(99)00186-x
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发表时间：
2000-04-01
期刊：
影响因子：
2.7
通讯作者：
Johnson, DR
中科院分区：
文献类型：
--
作者：
Johnson, DR
人I类MHC基因座HLA-A、-B和-C的表达用基因座特异性引物通过逆转录和竞争性PCR检测。这种方法允许通过分析扩增产物明确地鉴定靶序列。JY和Pala淋巴母细胞样B细胞表达HLA-A mRNA高于HLA-B mRNA，而HLA-C mRNA表达较少。Rail Burkitt淋巴瘤和HeLa癌细胞表达大约等量的HLA-A和HLA-C mRNA，但较少表达HLA-B mRNA。罐状滋养层细胞不表达HLA I类mRNA。令人惊讶的是，K562白血病细胞表达大量HLA-C mRNA。然而，K562细胞不含可检测到的HLA-A或-B mRNA，表明这些位点是独立调节的。此外，培养的内皮细胞和平滑肌细胞表达低的、近似等量的HLA-A、-B和-C mRNA，而供体匹配的EBV转化的B细胞表达更多的HLA-B mRNA，表明细胞类型依赖性调节是差异位点表达的基础。最后，HLA I类分子在细胞表面的表达与总的HLA mRNA相关，但与任何一个位点编码的mRNA无关。这些HLA I类基因座的差异表达可能通过优先向T细胞呈递不同的肽而有助于细胞类型依赖性免疫反应。(C)美国组织相容性和免疫遗传学学会，2000年。出版社：Elsevier Science Inc.
Expression of the human class I MHC loci, HLA-A, -B, and -C, was examined by reverse transcription and competitive PCR with locus-specific primers. This approach allows unambiguous identification of target sequences by analysis of the amplified products. JY and Pala lymphoblastoid B cells express more HLA-A than HLA-B mRNAs and little HLA-C mRNA. Rail Burkitt lymphoma and HeLa carcinoma cells express approximately equal amounts of HLA-A and HLA-C mRNAs but less HLA-B mRNA. Jar trophoblast cells express no HLA class I mRNAs. Surprisingly, K562 leukemia cells express significant amounts of HLA-C mRNA. However, K562 cells cont-ain no detectable HLA-A or -B mRNAs, suggesting that these loci are regulated independently. Furthermore, cultured endothelial cells and smooth muscle cells express low, approximately equal amounts of HLA-A, -B, and -C mRNAs, whereas donor-matched, EBV transformed B cells express much more HLA-B mRNA, suggesting that cell type dependent regulation underlies differential locus expression. Finally, expression of HLA class I molecules on che cell surface correlates with total HLA mRNAs but not with mRNAs encoded by any one locus. Differential expression of these HLA class I loci may contribute to cell-type dependent immune reactions by preferentially presenting distinct peptides to T cells. (C) American Society for Histocompatibility and Immunogenetics, 2000. Published by Elsevier Science Inc.