HD-ZIP IV gene Roc8 regulates the size of bulliform cells and lignin content in rice.
HD-ZIP IV gene Roc8 regulates the size of bulliform cells and lignin content in rice.
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HD-ZIP IV基因ROC8调节大米中的牛蛋白细胞和木质素含量的大小。
DOI：
10.1111/pbi.13435
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发表时间：
2020-12
影响因子：
13.8
通讯作者：
Zhang Z
中科院分区：
文献类型：
--
作者：
Sun J;Cui X;Teng S;Kunnong Z;Wang Y;Chen Z;Sun X;Wu J;Ai P;Quick WP;Lu T;Zhang Z
位于叶片上表皮的泡状细胞是影响叶片形态的重要细胞结构之一。虽然许多机制调节泡状细胞的发展已被报道，精细的调控机制管理这一过程很少被描述。为了鉴定调节水稻叶片形态的新组分，选择了从苗期到开花表现出组成型滚动表型的突变体crm 1-D进行进一步分析。crm 1-D的解剖分析归因于泡状细胞的尺寸减小。crm 1-D受一个显性核基因控制。基于图位的克隆显示，HD拉链IV家族成员Roc 8负责crm 1-D表型。值得注意的是，Roc 8基因3′-非翻译区（3′-UTR）中的50 bp序列在翻译水平上抑制Roc 8。此外，roc 8基因敲除株系显著增加了泡状细胞的大小。一系列的分析表明，Roc 8负调节泡状细胞的大小。出乎意料的是，还观察到Roc 8正介导木质素生物合成而不引起生产损失。上述结果表明Roc 8在培育高光合效率低木质素含量的材料上具有实际应用价值。
The morphology of bulliform cells located on the upper epidermis of leaves is one of the most important cell structures affecting leaf shape. Although many mechanisms regulating the development of bulliform cells have been reported, the fine regulatory mechanisms governing this process have rarely been described. To identify novel components regulating rice leaf morphology, a mutant showing a constitutively rolling phenotype from the seedling stage to flowering, known as crm1‐D, was selected for further analysis. Anatomical analyses in crm1‐D were attributable to the size reduction of bulliform cells. The crm1‐D was controlled by a single dominant nuclear gene. Map‐based cloning revealed that Roc8, an HD zipper class IV family member, was responsible for the crm1‐D phenotype. Notably, the 50‐bp sequence in the 3′‐untranslated region (3′‐UTR) of the Roc8 gene represses Roc8 at the translational level. Moreover, the roc8 knockdown lines notably increased the size of bulliform cells. A series of assays revealed that Roc8 negatively regulates the size of bulliform cells. Unexpectedly, Roc8 was also observed to positively mediate lignin biosynthesis without incurring a production penalty. The above results show that Roc8 may have a practical application in cultivating materials with high photosynthetic efficiency and low lignin content.
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