Metabolomics Reveals Phospholipids as Important Nutrient Sources during Salmonella Growth in Bile In Vitro and In Vivo
Metabolomics Reveals Phospholipids as Important Nutrient Sources during Salmonella Growth in Bile In Vitro and In Vivo
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DOI：
10.1128/jb.05132-11
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发表时间：
2011-09-01
影响因子：
3.2
通讯作者：
Finlay, B. Brett
中科院分区：
文献类型：
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作者：
Antunes, L. Caetano M.;Andersen, Sarah K.;Finlay, B. Brett
在宿主的定殖期间，细菌病原体面临许多挑战，这些挑战必须被克服以使定殖成功发生。这需要细菌对周围环境的感知和对所遇到的条件的适应。胆汁的抗菌作用是哺乳动物胃肠道病原体定植的主要障碍之一。鼠伤寒沙门氏菌对胆汁的耐药性有特殊的机制。此外，沙门氏菌可以成功地在胆汁中繁殖，将其用作营养来源。这一成就与发病机制高度相关，因为沙门氏菌定殖宿主的胆囊，在那里它可以无症状地携带并促进进一步的宿主传播和传播。为了深入了解沙门氏菌在胆汁中生长的机制，我们通过代谢组学方法研究了沙门氏菌在体外和体内生长期间胆汁化学组成的变化。我们的数据表明，磷脂是沙门氏菌在实验室生长期间以及小鼠胆囊感染期间碳和能量的重要来源。在这一领域的进一步研究将产生一个更好的了解沙门氏菌如何利用这种普遍敌对的环境为自己的利益。
During the colonization of hosts, bacterial pathogens are presented with many challenges that must be overcome for colonization to occur successfully. This requires the bacterial sensing of the surroundings and adaptation to the conditions encountered. One of the major impediments to the pathogen colonization of the mammalian gastrointestinal tract is the antibacterial action of bile. Salmonella enterica serovar Typhimurium has specific mechanisms involved in resistance to bile. Additionally, Salmonella can successfully multiply in bile, using it as a source of nutrients. This accomplishment is highly relevant to pathogenesis, as Salmonella colonizes the gallbladder of hosts, where it can be carried asymptomatically and promote further host spread and transmission. To gain insights into the mechanisms used by Salmonella to grow in bile, we studied the changes elicited by Salmonella in the chemical composition of bile during growth in vitro and in vivo through a metabolomics approach. Our data suggest that phospholipids are an important source of carbon and energy for Salmonella during growth in the laboratory as well as during gallbladder infections of mice. Further studies in this area will generate a better understanding of how Salmonella exploits this generally hostile environment for its own benefit.