Anti-inflammatory effect of a Nuphar lutea partially purified leaf extract in murine models of septic shock
Anti-inflammatory effect of a Nuphar lutea partially purified leaf extract in murine models of septic shock
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DOI：
10.1016/j.jep.2014.11.048
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发表时间：
2015-02-23
影响因子：
5.4
通讯作者：
Gopas, J.
中科院分区：
文献类型：
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作者：
Ozer, J.;Levi, T.;Gopas, J.
民族药理学的相关性：各种植物器官的Nuphar lutea（L.）SM.（睡莲科）在传统医学中用于治疗关节炎、发烧、疼痛、疼痛和炎症。本研究的主要目的是确定荷叶提取物（NUP）在两种感染性休克模型中的抗炎作用：（1）用致死剂量的LPS攻击小鼠的存活，测定血清中促炎和抗炎细胞因子以及细胞培养中的腹腔巨噬细胞。(2)NUP在粪便诱导的腹膜炎小鼠模型中的作用。材料和方法：NUP预处理在两种急性脓毒性休克模型中部分保护小鼠。我们的结论是，NUP是通过抑制NF-κ B B通路，调节细胞因子的产生和ERK磷酸化的抗炎作用。此外，NUP预处理减少了腹腔巨噬细胞核NE-κ B易位。NUP还降低了LPS处理小鼠血清或LPS刺激2-6 h的腹腔巨噬细胞上清液中促炎细胞因子TNF-α、IL-6和IL-12的产生。用NUP预处理引起抗炎细胞因子IL-10的显著增加。NUP预处理降低并延迟了粪便诱导的腹膜炎小鼠的死亡率。我们的研究还表明，NUP预处理诱导剂量依赖性磷酸化的ERK在腹腔巨噬细胞。由于大多数的报告有关的抗炎作用的Nuphar lutea指根茎和根粉末和提取物，重要的是要澄清叶提取物的有效性作为一个来源，这样的activity.Conclusion：NUP预处理部分保护小鼠在两个模型的急性感染性休克。我们的结论是，NUP是通过抑制NF-κ B通路，调节细胞因子的产生和ERK磷酸化的抗炎。(C)2014爱思唯尔爱尔兰有限公司版权所有。
Ethnopharmacological relevance: Various plant organs of Nuphar lutea (L.) SM. (Nymphaeaceae) are used in traditional medicine for the treatment of arthritis, fever, aches, pains and inflammation. The main purpose of this study was to determine the anti-inflammatory effect of Nuphar lutea leaf extract (NUP) in two septic shock models: (1) Survival of mice challenged with a lethal dose of LPS, determination of proinflammatory and anti-inflammatory cytokines in serum, as well as in peritoneal macrophages in cell culture. (2) The effect of NUP in a murine model of fecal-induced peritonitis.Materials and methods: NUP pre-treatment partially protected mice in two models of acute septic shock. We concluded that NUP is anti-inflammatory by inhibiting the NF-kappa B pathway, modulating cytokine production and ERK phosphorylation.Results: A significant average survival rate (60%) of LPS lethally-challenged mice was achieved by pretreatment with NUP. In addition, NUP pre-treatment reduced nuclear NE-kappa B translocation in peritoneal macrophages. The production of pro-inflammatory cytokines, TNF-alpha, IL-6 and IL-12, in the sera of LPS-treated mice or in the supernatants of peritoneal macrophages stimulated with LPS for 2-6 h was also decreased by NUP. Pre-treatment with NUP caused a significant increase in the anti-inflammatory cytokine IL-10. The NUP pre-treatment reduced and delayed mortality in mice with fecal-induced peritonitis. Our studies also revealed that NUP pre-treatment induced a dose-dependent phosphorylation of ERK in peritoneal macrophages. Since most of the reports about the anti-inflammatory effect of Nuphar lutea refer to rhizome and root powder and extracts, it is important to clarify the effectiveness of leaf extract as a source for such activity.Conclusion: NUP pre-treatment partially protected mice in two models of acute septic shock. We concluded that NUP is anti-inflammatory by inhibiting the NF-kappa B pathway, modulating cytokine production and ERK phosphorylation. (C) 2014 Elsevier Ireland Ltd. All rights reserved.