RNA-directed DNA polymerase and virus-induced leukemia in mice.
RNA-directed DNA polymerase and virus-induced leukemia in mice.
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RNA 指导的 DNA 聚合酶和病毒诱导的小鼠白血病。
DOI：
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发表时间：
1973
影响因子：
11.1
通讯作者：
R. Gallo
中科院分区：
文献类型：
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作者：
A. Wu;R. Ting;R. Gallo
利福霉素抗生素可抑制Rauscher白血病病毒的RNA指导的DNA聚合酶，防止病毒的致白血病活性，并且致白血病的作用与纯化酶的抑制程度相关。利福霉素SV衍生物对酶活性的这种抑制是由于酶和抑制剂之间相对紧密的结合，然而这种结合可以被非离子去污剂逆转。这项研究的结果表明，RNA指导的DNA聚合酶是必不可少的诱导白血病的外源性病毒，并与以前的观察，相同的衍生物在体外阻断病毒转化。
Rifamycin antibiotics, which inhibit RNA-directed DNA polymerase of Rauscher leuckemia virus, prevent the leukemogenic activity of the virus, and the effect of leukemogenesis correlates with the magnitude of inhibition of the purified enzyme. This inhibition of enzyme activity by rifamycin SV derivatives is due to a relatively tight binding between the enzymes and the inhibitors, yet the binding can be reversed by nonionic detergent. The results of this study suggest that RNA-directed DNA polymerase is essential for induction of leukemia by exogenous virus and correlate with the previous observation that the same derivatives block viral transformation in vitro.