CTCF terminal segments are unstructured
CTCF terminal segments are unstructured
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DOI：
10.1002/pro.367
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发表时间：
2010-05-01
期刊：
影响因子：
8
通讯作者：
Miranda, J. J. L.
中科院分区：
文献类型：
--
作者：
Martinez, Selena R.;Miranda, J. J. L.
人CCCTC结合因子CTCF通过将远端DNA元件共定位在相同甚至不同的染色体上来组织和调节基因组的转录。这种蛋白质由11个锌指组成，两侧是结构和功能未知的多肽片段。我们纯化了重组末端片段，并观察到两者都是延伸的，单体的，主要由无序的内容。因此，我们推测，终端延伸的作用，也许是所有的CTCF，是作为一个支架上的其他蛋白质的组装在一个特定的结合位点。
The human CCCTC-binding factor, CTCF, organizes and regulates transcription of the genome by colocalizing distant DNA elements on the same and even different chromosomes. This protein consists of 11 zinc fingers flanked by polypeptide segments of unknown structure and function. We purified recombinant terminal fragments and observed that both are extended, monomeric, and predominantly consist of unordered content. We thus speculate that the role of the terminal extensions, and perhaps all of CTCF, is to act as a scaffold for the assembly of other proteins on a specific binding site.